Affinity purified from pooled serum. Learn more.

Connexin43 (Ser368) Antibody

Catalog #: p1007-368 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Zebra fish, Canine, Guinea Pig
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GJA1
Molecular Reference:
~43 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1007-368, RRID:AB_2492064
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin43.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Synonyms: ExpandCollapse
  • Connexin 43 antibody
  • Connexin-43 antibody
  • Cx 43 antibody
  • Cx43 antibody
  • CXA1_HUMAN antibody
  • DFNB38 antibody
  • Gap junction 43 kDa heart protein antibody
  • Gap junction alpha-1 protein antibody
  • Gap junction protein alpha 1 43kDa (connexin 43) antibody
  • Gap junction protein alpha 1 43kDa antibody
  • Gap junction protein alpha like antibody
  • GJA 1 antibody
  • Gja1 antibody
  • GJAL antibody
  • ODD antibody
  • ODDD antibody
  • ODOD antibody
  • SDTY3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.

Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.

Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.

Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.

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Connexin43 ser368 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43 kDa connexin43 phosphorylated at Ser368 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

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