Affinity purified from pooled serum. Learn more.

Connexin43 Antibody

Name: Connexin43 Antibody
SKU: 327-CON
Price: 365 USD
Availability: InStock

Catalog #: 327-CON Category: Cardiovascular Datasheet:

$109.00 – $365.00

Rabbit polyclonal antibody

Pooled Serum

Formulation:: Affinity Purified from Pooled Serum
Species Reactivity:: Rat, Mouse, Human, Xenopus, Chicken, Non-human primate, Zebra fish, Sheep, Canine
Applications:: WB 1:1000
Species:: Rabbit
Gene Name:: GJA1
Molecular Reference:: ~43 kDa
Cite This Antibody:: PhosphoSolutions Cat# 327-CON, RRID:AB_2492063

Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.

The antibody is prepared from pooled rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser³⁶⁸ by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser³⁶⁸ by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Synonyms: ExpandCollapse

Connexin 43 antibody
Connexin-43 antibody
Cx 43 antibody
Cx43 antibody
CXA1_HUMAN antibody
DFNB38 antibody
Gap junction 43 kDa heart protein antibody
Gap junction alpha-1 protein antibody
Gap junction protein alpha 1 43kDa (connexin 43) antibody
Gap junction protein alpha 1 43kDa antibody
Gap junction protein alpha like antibody
GJA 1 antibody
Gja1 antibody
GJAL antibody
ODD antibody
ODDD antibody
ODOD antibody
SDTY3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.

Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.

Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.

Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.