Collagen I, alpha 1, telopeptide Antibody

Catalog #: 322-COLT Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Formulation:
Affinity purified
100% Homology:
Rat, Mouse, Human, Sheep
Applications:
WB 1:1,000IHC 1:100
Species:
Rabbit
Gene Name:
COL1A1
Molecular Reference:
~140 kDa
Cite This Antibody:
PhosphoSolutions Cat# 322-COLT, RRID:AB_2492061
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide from the C-terminal region of human collagen I α1 telopeptide sequence.

The antibody is affinity purified.

Biological Significance: ExpandCollapse

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).

Storage

Liquid in PBS. Contains no preservative.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C up to six months after date of receipt. Avoid freeze/thaw cycles.

Product Specific References

Gatto, R.G., Li, W., Gao, J. and Magin, R.L., 2018. In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR in Biomedicine, Aug;31(8):e3954. PMID: 30117615

Reese C, Lee R, Bonner M, Perry B, Heywood J, Silver RM, Tourkina E, Visconti RP, Hoffman S. (2014) Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry. Front Pharmacol. 2014 Jun 16;5:141. PMID: 24999331

Bains, SN, Tourkina E, Atkinson C, Joseph K, Tholanikunnel B, Chu HW, Riemer EC, Martin R, Hoffman S (2012) Loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma. Allergy. 2012 Dec;67(12): 1601-4. PMID: 23004679

Wessels A, van den Hoff MJ, Adamo RF, Phelps AL, Lockhart MM, Sauls K, Briggs LE, Norris RA, van Wijk B, Perez-Pomares JM, Dettman RW, Burch JB. (2012) Epicardially derived fibroblasts preferentially contribute to the parietal leaflets of the atrioventricular valves in the murine heart. Dev Biol. 2012 Jun 15;366(2): 111-24. PMID: 22546693

Collagen I, alpha 1, telopeptide Antibody
Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.
Collagen I, alpha 1, telopeptide Antibody
Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.

You may also like…