Collagen I, alpha 1, propeptide Antibody

Catalog #: 321-COLP Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Rat, Mouse, Human
Species Reactivity:
Avian, Amphibians, Most Mammals
Applications:
WB 1:1000IHC 1:100 Don't see your application?
Species:
Rabbit
Gene Name:
COL1A1
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# 321-COLP, RRID:AB_2492060
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the human collagen I α1 propeptide sequence.

The antibody is affinity purified.

Biological Significance: ExpandCollapse

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005)

Synonyms: ExpandCollapse
  • Alpha 1 type I collagen antibody
  • Alpha 2 type I collagen antibody
  • alpha 2 type I procollagen antibody
  • alpha 2(I) procollagen antibody
  • alpha 2(I)-collagen antibody
  • Alpha-1 type I collagen antibody
  • alpha1(I) procollagen antibody
  • CO1A1_HUMAN antibody
  • COL1A1 antibody
  • COL1A2 antibody
  • collagen alpha 1 chain type I antibody
  • Collagen alpha-1(I) chain antibody
  • collagen alpha-1(I) chain preproprotein antibody
  • Collagen I alpha 1 polypeptide antibody
  • Collagen I alpha 2 polypeptide antibody
  • collagen of skin, tendon and bone, alpha-1 chain antibody
  • collagen of skin, tendon and bone, alpha-2 chain antibody
  • Collagen type I alpha 1 antibody
  • Collagen type I alpha 2 antibody
  • EDSC antibody
  • OI1 antibody
  • OI2 antibody
  • OI3 antibody
  • OI4 antibody
  • pro-alpha-1 collagen type 1 antibody
  • type I proalpha 1 antibody
  • type I procollagen alpha 1 chain antibody
  • Type I procollagen antibody

Storage

100 µl in PBS. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse
Flow Cytometry: Mouse


Flow Cytometry: Mouse
PMID Dilution Publication
24999331 1.67ug/ml Reese, C., et al. 2014 Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry. Front Pharmacol. 2014 Jun 16;5:141.


Western blot: Mouse
PMID Dilution Publication
24999331 0.2ug/ml Reese, C., et al. 2014 Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry. Front Pharmacol. 2014 Jun 16;5:141.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Collagen I, alpha 1, propeptide Antibody
Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.

Click here to view our Western blotting and lysate preparation protocols.
Collagen I, alpha 1, propeptide Antibody
Immunostaining of fibrotic mouse lung tissue showing specific staining of collagen I molecules (Cat. #621-COLP, 1:100, red) that are still associated with the cells in which they were synthesized. The blue is staining DNA.

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