COBRA1 (NELF B) Antibody

Catalog #: 319-COBRA Category: Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Reactivity:
Rat, Mouse, Human
Applications:
WB 1:1000
Species:
Mouse
Gene Name:
COBRA1
Molecular Weight:
~62 kDa
Cite This Antibody:
PhosphoSolutions Cat# 319-COBRA, RRID:AB_2492059
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human COBRA1 purified from E. coli.

The antibody is protein G purified culture supernatant.

Biological Significance: ExpandCollapse

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ER?) and repress ER?-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Synonyms: ExpandCollapse
  • A730008L03Rik antibody
  • AB041607 antibody
  • AI663983 antibody
  • Cofactor of BRCA1 antibody
  • DKFZp586B0519 antibody
  • KIAA1182 antibody
  • Negative elongation factor B antibody
  • Negative elongation factor protein B antibody
  • Nelf b antibody
  • NELF-B antibody
  • NELFB antibody
  • RGD1307832 antibody
  • RP13-122B23.3 antibody
  • RP23-132N23.4 antibody

Storage

100 µl in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134– 46.

Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun.325 (2): 568– 73.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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COBRA1 (NELF B) Antibody
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.

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