COBRA1 (NELF B) Antibody

Our mouse monoclonal COBRA1 (NELFB) total protein antibody is Protein G purified. It is excellent for use in WB.

Catalog #: 319-COBRA Categories: , Datasheet:


  • SizePrice
Protein G Purified
Species Tested:
Rat, Mouse, Human
WB 1:1000 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
Molecular Weight:
~62 kDa
Cite This Antibody:
PhosphoSolutions Cat# 319-COBRA, RRID:AB_2492059
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human COBRA1 purified from E. coli.

The antibody is protein G purified culture supernatant.

Biological Significance: ExpandCollapse

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Synonyms: ExpandCollapse
  • A730008L03Rik antibody
  • AB041607 antibody
  • AI663983 antibody
  • Cofactor of BRCA1 antibody
  • DKFZp586B0519 antibody
  • KIAA1182 antibody
  • Negative elongation factor B antibody
  • Negative elongation factor protein B antibody
  • Nelf b antibody
  • NELF-B antibody
  • NELFB antibody
  • RGD1307832 antibody
  • RP13-122B23.3 antibody
  • RP23-132N23.4 antibody


100 µl in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134–46. PMID: 15342491

Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun. 325 (2): 568–73.PMID: 15530430

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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