CNP (CNPase) Antibody

Our rabbit polyclonal CNP total protein antibody is neat serum. It is a great marker for oligodendrocytes and Schwann cells in WB and IHC.

Catalog #: 325-CNP Categories: , , , Datasheet:

$325.00

Size:
50 µl
Formulation:
Neat serum
Species Tested:
Human, Mouse, Rat, Sheep
Applications:
WB 1:1000IHC 1:500-1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
CNP
Molecular Weight:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# 327-CON, RRID:AB_2492063
Antigen/Purification: ExpandCollapse

The antigen is an endogenous rabbit 2,3-cyclic nucleotide-3-phospho-diesterase.

The antibody is neat serum.

Biological Significance: ExpandCollapse

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Synonyms: ExpandCollapse
  • 2” antibody
  • 2’3′ cyclic nucleotide 3′ phosphodiesterase antibody
  • 3”-cyclic-nucleotide 3”-phosphodiesterase antibody
  • CN37_HUMAN antibody
  • CNP 1 antibody
  • CNP antibody
  • CNP1 antibody
  • CNPase antibody

Storage

50 ul neat serum.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunohistochemistry

Rat Brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat
Western Blot: Mouse


Immunohistochemistry: Rat
PMID Dilution Publication
2207709 1:1000 Ramaswamy, S.G., et al. 1990 A novel autoantibody from a rabbit preimmune serum that immunostains myelinated nerves of the brain. Brain research bulletin, 25(1), pp.193-197.


Western Blot: Mouse
PMID Dilution Publication
29461205 1:2000 González-Fernández, E., et al. 2018 PTEN negatively regulates the cell lineage progression from NG2+ glial progenitor to oligodendrocyte via mTOR-independent signaling. eLife, 7, p.e32021.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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