Choline Acetyltransferase Antibody, Rabbit

Our rabbit polyclonal Choline Acetyltransferase total protein antibody is neat serum. It is an excellent cholinergic neuronal marker for use in WB and IHC.

Catalog #: 316-ChAT Categories: , , , , Datasheet:


  • SizePrice
Neat serum
Species Tested:
Human, Mouse, Rat, Avian, Chicken, Guinea Pig, Non-Human Primate
WB 1:1000IHC 1:100 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~68/70 kDa
Cite This Antibody:
PhosphoSolutions Cat# 316-ChAT, RRID:AB_2492056
Antigen/Purification: ExpandCollapse

The antigen is a native choline acetyltransferase purified from human placenta.

The antibody is neat serum.

Biological Significance: ExpandCollapse

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer’s disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Synonyms: ExpandCollapse
  • Acetyl CoA choline O acetyltransferase antibody
  • Acetyl CoA:choline O acetyltransferase antibody
  • ChAT antibody
  • CHOACTase antibody
  • Choline acetylase antibody
  • choline acetyltransferase antibody
  • Choline O acetyltransferase antibody
  • Choline O-acetyltransferase antibody
  • CLAT_HUMAN antibody
  • CMS1A antibody
  • CMS1A2 antibody
  • EC antibody
  • OTTHUMP00000019583 antibody
  • OTTHUMP00000019584 antibody


100 µl neat serum. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459. PMID: 8434662

Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31. PMID: 10613493

Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123. PMID: 10488813

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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