Choline Acetyltransferase Antibody, Rabbit

Catalog #: 316-ChAT Categories: , , , Datasheet:

$109.00$365.00

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Cholinergic neuronal marker

Formulation:
Neat serum
Specificity:
Rat, Mouse, Human, Chicken, Non-human primate, Guinea Pig
Applications:
WB 1:1,000IHC 1:100
Species:
Rabbit polyclonal
Gene Name:
CHAT
Molecular Reference:
~68/70 kDa
Cite This Antibody:
PhosphoSolutions Cat# 316-ChAT, RRID:AB_2492056
Antigen/Purification: ExpandCollapse

The antigen is a native choline acetyltransferase purified from human placenta.

The antibody is neat serum.

Biological Significance: ExpandCollapse

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer’s disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Storage

100 µl neat serum.. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.

Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.

Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.

Choline Acetyltransferase Antibody, Rabbit
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68/70 kDa ChAT.

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