Choline Acetyltransferase Antibody, Goat

Catalog #: 315-ChAT Categories: , , , Datasheet:


  • SizePrice

Cholinergic neuronal marker for IHC and Western Blot!


Affinity Purified
Species Reactivity:
Rat, Mouse, Human, Chicken, Non-human primate, Guinea Pig
WB 1:100IHC 1:100
Goat polyclonal
Gene Name:
Molecular Weight:
~70 kDa
Cite This Antibody:
PhosphoSolutions Cat# 315-CHAT, RRID:AB_2492055
Antigen/Purification: ExpandCollapse

The antigen is recombinant human choline acetyltransferase.

The antibody is prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.

Biological Significance: ExpandCollapse

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer’s disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Synonyms: ExpandCollapse
  • Acetyl CoA choline O acetyltransferase antibody
  • Acetyl CoA:choline O acetyltransferase antibody
  • ChAT antibody
  • CHOACTase antibody
  • Choline acetylase antibody
  • choline acetyltransferase antibody
  • Choline O acetyltransferase antibody
  • Choline O-acetyltransferase antibody
  • CLAT_HUMAN antibody
  • CMS1A antibody
  • CMS1A2 antibody
  • EC antibody
  • OTTHUMP00000019583 antibody
  • OTTHUMP00000019584 antibody


100 µl in PBS + 5 mg/ml BSA + 0.2% NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Reference

Blandford, S.N., Hooper, M.L., Yabana, T., Chauhan, B.C., Baldridge, W.H. and Farrell, S.R., 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative ophthalmology & visual science, 60(1), pp.183-191. PMID: 30640971

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Choline Acetyltransferase Antibody, Goat
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.
Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.

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