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Choline Acetyltransferase Antibody, chicken

Chicken Polyclonal Antibody from Aves Labs

Catalog #: ChAT Categories: , , , , , Datasheet:

$399.00

Size:
1000 µl
Formulation:
Affinity Purified IgY
Species Tested:
Mouse
Expected Reactivity:
Human, Mouse, Rat
Applications:
IHC 1:1000-1:2000ICC 1:1000-1:2000
Host Species:
Chicken Polyclonal
Gene Name:
CHAT
Molecular Weight:
~70 kDa
Cite This Antibody:
Aves Labs Cat# CHAT, RRID:AB_2313537
Antigen/Purification: ExpandCollapse

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Synonyms: ExpandCollapse
  • Acetyl CoA choline O acetyltransferase antibody
  • Acetyl CoA:choline O acetyltransferase antibody
  • ChAT antibody
  • CHOACTase antibody
  • Choline acetylase antibody
  • choline acetyltransferase antibody
  • Choline O acetyltransferase antibody
  • Choline O-acetyltransferase antibody
  • CLAT_HUMAN antibody
  • CMS1A antibody
  • CMS1A2 antibody
  • EC 2.3.1.6 antibody
  • OTTHUMP00000019583 antibody
  • OTTHUMP00000019584 antibody

Storage

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Store at 4˚C in the dark. Under these conditions, the antibody should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

Product Specific References

Wenze Niu, Tong Zang, Derek K. Smith, Tou Yia Vue, Yuhua Zou, Robert Bachoo, Jane E. Johnson, and Chun-Li Zhang. (2015), ‘SOX2 Reprograms Resident Astrocytes into Neural Progenitors in the Adult Brain.’ Stem Cell Reports. 10.1016/j.stemcr.2015.03.006.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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