Affinity purified from pooled serum. Learn more.

Che-1 (AATF) (Ser477) Antibody

We are the original manufacturer of our Che-1 (AATF) (Ser477) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p142-477 Categories: , , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Expected Reactivity:
Rat, Mouse, Bovine, Canine, Non-human primate, Sheep, Xenopus
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~66 kDa
Cite This Antibody:
PhosphoSolutions Cat# p142-477, RRID:AB_2492057
Antigen/Purification: ExpandCollapse

The antigen is a synthetic phospho-peptide surrounding the phospho Ser477 of human Che-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Synonyms: ExpandCollapse
  • AATF antibody
  • AATF_HUMAN antibody
  • Apoptosis antagonizing transcription factor antibody
  • Apoptosis-antagonizing transcription factor antibody
  • BFR2 antibody
  • CHE 1 antibody
  • CHE1 antibody
  • DED antibody
  • Protein AATF antibody
  • Rb binding protein Che 1 antibody
  • Rb-binding protein Che-1 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
27012205not listed Liu, M., et al. 2016 Che-1 gene silencing induces osteosarcoma cell apoptosis by inhibiting mutant p53 expression. Biochemical and biophysical research communications, 473(1), pp.168-173.
25996291not listed Desantis, A., et al. 2015 Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell death & disease, 6(5), p.e1764.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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