Affinity purified from pooled serum. Learn more.

Che-1 (AATF) (Ser477) Antibody

Catalog #: p142-477 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Bovine, Non-human primate, Sheep, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
AATF
Molecular Reference:
~66 kDa
Cite This Antibody:
PhosphoSolutions Cat# p142-477, RRID:AB_2492057
Antigen/Purification: ExpandCollapse

The antigen is a synthetic phospho-peptide surrounding the phospho Ser477 of human Che-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Synonyms: ExpandCollapse
  • AATF antibody
  • AATF_HUMAN antibody
  • Apoptosis antagonizing transcription factor antibody
  • Apoptosis-antagonizing transcription factor antibody
  • BFR2 antibody
  • CHE 1 antibody
  • CHE1 antibody
  • DED antibody
  • Protein AATF antibody
  • Rb binding protein Che 1 antibody
  • Rb-binding protein Che-1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Desantis, A., Bruno, T., Catena, V., De Nicola, F., Goeman, F., Iezzi, S., Sorino, C., Gentileschi, M.P., Germoni, S., Monteleone, V. and Pellegrino, M., 2015. Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell death & disease, 6(5), p.e1764. PMID: 25996291

Liu, M., Wang, D. and Li, N., 2016. Che-1 gene silencing induces osteosarcoma cell apoptosis by inhibiting mutant p53 expression. Biochemical and biophysical research communications, 473(1), pp.168-173. PMID: 27012205

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Che-1 (AATF) ser477 Antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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