Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- 100% Homology:
- Rat, Mouse, Human, Xenopus, Bovine, Non-human primate, Sheep, Canine
- WB 1:1,000
- Gene Name:
- Molecular Reference:
- ~66 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p142-477, RRID:AB_2492057
The antigen is a synthetic phospho-peptide surrounding the phospho Ser477 of human Che-1.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).