c-FOS Antibody

Catalog #: 309-cFOS Categories: , , , Datasheet:

$109.00$365.00

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Great for staining the nuclei of activated neurons!

 

Formulation:
Protein G purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:1000ICC 1:1000
Species:
Mouse Monoclonal
Gene Name:
FOS
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 309-cFOS, RRID:AB_2632380
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human c-FOS expressed in and purified from E. Coli.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

c-FOS is a member of the FOS transcription factor family which forms dimers with c-JUN to produce the Activator Protein 1 (AP-1) complex which plays a key role in critical cellular processes such as cell proliferation, differentiation and apoptosis (Chiu et al., 1988).  c-FOS expression has been demonstrated to be a useful marker of neuronal activation as it is rapidly induced following various stimuli (Hoffman et al., 1993).  Additionally, c-FOS has been shown to be overexpressed in a variety of malignant tumor types (Milde-Langosch 2005).

Synonyms: ExpandCollapse
  • Activator protein 1 antibody
  • AP 1 antibody
  • C FOS antibody
  • Cellular oncogene c fos antibody
  • Cellular oncogene fos antibody
  • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
  • FBJ murine osteosarcoma viral oncogene homolog antibody
  • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
  • FBJ Osteosarcoma Virus antibody
  • FOS antibody
  • FOS protein antibody
  • FOS_HUMAN antibody
  • G0 G1 switch regulatory protein 7 antibody
  • G0/G1 switch regulatory protein 7 antibody
  • G0S7 antibody
  • Oncogene FOS antibody
  • p55 antibody
  • proto oncogene c Fos antibody
  • Proto oncogene protein c fos antibody
  • Proto-oncogene c-Fos antibody
  • v fos FBJ murine osteosarcoma viral oncogene homolog antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HeLa Cells

Immunohistochemistry

Rat Hippocampus
Mouse Brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Chiu, R., Boyle, W. J., Meek, J., Smeal, T., Hunter, T., & Karin, M. (1988). The c-fos protein interacts with c-junAP-1 to stimulate transcription of AP-1 responsive genes. Cell, 54(4), 541-552.

Hoffman, G. E., Smith, M. S., & Verbalis, J. G. (1993). c-Fos and related immediate early gene products as markers of activity in neuroendocrine systems. Frontiers in neuroendocrinology, 14(3), 173-213.

Milde-Langosch, K. (2005). The Fos family of transcription factors and their role in tumourigenesis. European journal of cancer, 41(16), 2449-2461.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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cFOS antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (catalog # 309-cFOS, 1:1000, green) while Anti-Vimentin (cat #2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).

Download the protocol used for this image:

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