c-FOS Antibody

Catalog #: 309-cFOS Categories: , , , Datasheet:

$109.00$365.00

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Great for staining the nuclei of activated neurons!

Formulation:
Protein G purified
Specificity:
Bovine, Human, Mouse, Rat
Applications:
WB 1:2000IF 1:1000IHC 1:1000
Species:
Mouse Monoclonal
Gene Name:
FOS
Molecular Reference:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 309-cFOS, RRID:AB_2632380
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human c-FOS expressed in and purified from E. Coli.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

c-FOS is a member of the FOS transcription factor family which forms dimers with c-JUN to produce the Activator Protein 1 (AP-1) complex which plays a key role in critical cellular processes such as cell proliferation, differentiation and apoptosis (Chiu et al., 1988).  c-FOS expression has been demonstrated to be a useful marker of neuronal activation as it is rapidly induced following various stimuli (Hoffman et al., 1993).  Additionally, c-FOS has been shown to be overexpressed in a variety of malignant tumor types (Milde-Langosch 2005).

Storage

100 µl in PBS + 5 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General References

Chiu, R., Boyle, W. J., Meek, J., Smeal, T., Hunter, T., & Karin, M. (1988). The c-fos protein interacts with c-junAP-1 to stimulate transcription of AP-1 responsive genes. Cell, 54(4), 541-552.

Hoffman, G. E., Smith, M. S., & Verbalis, J. G. (1993). c-Fos and related immediate early gene products as markers of activity in neuroendocrine systems. Frontiers in neuroendocrinology, 14(3), 173-213.

Milde-Langosch, K. (2005). The Fos family of transcription factors and their role in tumourigenesis. European journal of cancer, 41(16), 2449-2461.

cFOS antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.

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