Affinity purified from pooled serum. Learn more.

cdc2 (Tyr15) Antibody

Catalog #: p135-15 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Xenopus
Species Reactivity:
Rat, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
CDK1
Molecular Weight:
~34 kDa
Cite This Antibody:
PhosphoSolutions Cat# p135-15, RRID:AB_2492052
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat cdc2.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).

Synonyms: ExpandCollapse
  • Cdc 2 antibody
  • Cdc2 antibody
  • CDC28A antibody
  • CDK-1 antibody
  • CDK 1 antibody
  • CDK1 antibody
  • CDK1_HUMAN antibody
  • CDKN1 antibody
  • CELL CYCLE CONTROLLER CDC2 antibody
  • Cell division control protein 2 antibody
  • Cell division control protein 2 homolog antibody
  • Cell division cycle 2 G1 to S and G2 to M antibody
  • Cell division protein kinase 1 antibody
  • Cell Divsion Cycle 2 Protein antibody
  • Cyclin Dependent Kinase 1 antibody
  • Cyclin-dependent kinase 1 antibody
  • DKFZp686L20222 antibody
  • MGC111195 antibody
  • p34 Cdk1 antibody
  • p34 protein kinase antibody
  • P34CDC2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. Storage and Stability.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

 

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Dranovsky, A., Vincent, I., Gregori, L., Schwarzman, A., Colflesh, D., Enghild, J., Strittmatter, W., Davies, P. and Goldgaber, D., 2001. Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer’s disease pathology. Neurobiology of aging, 22(4), pp.517-528. PMID: 11445251

Konishi, Y. and Bonni, A., 2003. The E2F–Cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. Journal of Neuroscience, 23(5), pp.1649-1658. PMID: 12629169

Maller JL (1991) Mitotic control. Curr Opin Cell Biol 3:269-275. PMID: 1883620

Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K. and Fukui, M., 2002. Suppression of Cdc2 Dephosphorylation at the Tyrosine 15 Residue during Nitrosourea-induced G 2 M phase Arrest in Glioblastoma Cell Lines. Journal of neuro-oncology, 59(1), pp.7-13. PMID: 12222840

Okano, H.J., Pfaff, D.W. and Gibbs, R.B., 1996. Expression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain. Developmental neuroscience, 18(3), pp.199-209. PMID: 8894448

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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cdc2 tyr15 Antibody
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, λ-Ptase (1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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