Affinity purified from pooled serum. Learn more.

Catenin, β- (Ser33,37) Antibody

Catalog #: p120-3337 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Expected Reactivity:
Rat, Xenopus
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Molecular Weight:
~83 kDa
Cite This Antibody:
PhosphoSolutions Cat# p120-3337, RRID:AB_2492049
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser33,37 of human β-catenin.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

β-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of β-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37and Thr41 by glycogen synthase kinase 3β (GSK3β) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of β-catenin. Mutations in components of this phosphorylation regulated process that prevent β-catenin hyperphosphorylation by GSK3β are strongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).

Synonyms: ExpandCollapse
  • Beta catenin antibody
  • Beta-catenin antibody
  • Cadherin associated protein antibody
  • Catenin (cadherin associated protein), beta 1, 88kDa antibody
  • Catenin beta 1 antibody
  • Catenin beta-1 antibody
  • CATNB antibody
  • CHBCAT antibody
  • CTNB1_HUMAN antibody
  • CTNNB antibody
  • CTNNB1 antibody
  • DKFZp686D02253 antibody
  • FLJ25606 antibody
  • FLJ37923 antibody
  • OTTHUMP00000162082 antibody
  • OTTHUMP00000165222 antibody
  • OTTHUMP00000165223 antibody
  • OTTHUMP00000209288 antibody
  • OTTHUMP00000209289 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
29184203 1:1000 Jung, E.M., et al. 2017 Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior. Nature Neuroscience, Dec;20(12):1694-1707.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Catenin, β- ser 33,37 Antibody
Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83 kDa β-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan β-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3β knocked down by SiRNA.

Click here to view our Western blotting and lysate preparation protocols.

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