Affinity purified from pooled serum. Learn more.

Catenin, β- (Ser33,37) Antibody

Catalog #: p120-3337 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Xenopus
Applications:
WB 1:1000
Species:
Rabbit
Molecular Reference:
~83 kDa
Cite This Antibody:
PhosphoSolutions Cat# p120-3337, RRID:AB_2492049
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser33,37 of human β-catenin.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

β-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of β-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37and Thr41 by glycogen synthase kinase 3β (GSK3β) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of β-catenin. Mutations in components of this phosphorylation regulated process that prevent β-catenin hyperphosphorylation by GSK3β are strongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific Reference

Jung, E.M., Moffat, J.J., Liu, J., Dravid, S.M., Gurumurthy, C.B. and Kim, W.Y., 2017. Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior. Nature Neuroscience, Dec;20(12):1694-1707. PMID: 29184203

 

Catenin, β- ser 33,37 Antibody
Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83 kDa β-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan β-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3β knocked down by SiRNA.

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