Affinity purified from pooled serum. Learn more.

CaM Kinase II (Thr306) Antibody

We are the original manufacturer of our Cam Kinase II (Thr306) rabbit polyclonal phospho-
specific antibody, affinity purified from pooled serum. Optimized in WB, IHC, and ICC.

Catalog #: p1005-306 Categories: , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Human, Xenopus, Zebra fish
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~50 kDa and ~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1005-306, RRID:AB_2560940
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr306 found in rat brain CaM Kinase II.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). CaMKIIα autophosphorylation at Thr286 and Thr305/Thr306 has recently been shown to regulate kinase activity and modulate subcellular targeting and is critical for normal synaptic plasticity and learning and memory (Baucum et al., 2015).

Synonyms: ExpandCollapse


  • Calcium/calmodulin dependent protein kinase II alpha antibody
  • Calcium/calmodulin dependent protein kinase II beta antibody
  • Calcium/calmodulin dependent protein kinase II delta antibody
  • Calcium/calmodulin dependent protein kinase II gamma antibody
  • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
  • CaM kinase II alpha antibody
  • CaM kinase II antibody
  • CaM kinase II beta antibody
  • CaM kinase II delta antibody
  • CaM kinase II gamma antibody
  • CaM kinase II subunit alpha antibody
  • CaMK-II subunit alpha antibody
  • CAMK2 antibody
  • Camk2a antibody
  • CAMK2B antibody
  • CAMK2D antibody
  • CAMK2G antibody
  • CAMKA antibody
  • KCC2A_HUMAN antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Baucum, A.J., Shonesy, B.C., Rose, K.L. and Colbran, R.J., 2015. Quantitative proteomics analysis of CaMKII phosphorylation and the CaMKII interactome in the mouse forebrain. ACS chemical neuroscience, 6(4), pp.615-631. PMID: 25650780

Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003) Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 6:217-218. PMID: 12563265

Meng FJ, Guo J, Zhang QG, Song B, Zhang GY (2003) Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 967:161-169. PMID: 12650977

Hudmon A, Schulman H (2002) Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 71:473-510. PMID: 12045104

Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ (2002) Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 36:493-505. PMID: 12408851

Soderling TR (2000) CaM-kinases: modulators of synaptic plasticity. Curr Opin Neurobiol 10:375-380. PMID: 10851169

Kennedy MB (1998) Signal transduction molecules at the glutamatergic postsynaptic membrane. Brain Res Rev 26:243-257. PMID: 9651538

Picciotto MR, Czernik AJ, Nairn AC (1993) Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. J Biol Chem 268:26512-26521. PMID: 8253780

Schulman H, Hanson PI (1993) Multifunctional Ca2+/calmodulin-dependent protein kinase. Neurochem Res 18:65-77. PMID: 8385278

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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