Calretinin Antibody

Catalog #: 303-CAL Categories: , , , Datasheet:

$109.00$365.00

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Clear

Excellent for use in IHC!

 

Formulation:
Protein G purified
Species Reactivity:
Rat, Mouse, Human, Non-human primate
Applications:
WB 1:1000IF 1:500IHC 1:500
Species:
Sheep polyclonal
Gene Name:
CALB2
Molecular Reference:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# 303-CAL, RRID:AB_2492050
Antigen/Purification: ExpandCollapse

The antigen is a  purified guinea pig calretinin.

The antibody is protein G purified sheep serum.

Biological Significance: ExpandCollapse

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).

Synonyms: ExpandCollapse
  • 29 kDa calbindin antibody
  • CAB 29 antibody
  • CAB29 antibody
  • CAL 2 antibody
  • CAL2 antibody
  • CALB 2 antibody
  • CALB2 antibody
  • CALB2_HUMAN antibody
  • Calbindin 2 29kDa antibody
  • Calbindin 2 antibody
  • Calbindin D29K antibody
  • Calbindin2 antibody
  • Calretinin antibody
  • CR antibody

Storage

100 µl in 10 mM Tris, 100 mM NaCl. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

General References

Attanoos RL, Dojcinov SD, Webb R, Gibbs AR. (2000) Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology.37(3):224-31.

Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.

Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.

Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.

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Calretinin Antibody
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.
Calretinin Antibody
Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.

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