Calretinin Antibody

Our sheep polyclonal Calretinin antibody is Protein G purified. It is excellent for use in WB, IHC, and IF.

Catalog #: 303-CAL Categories: , , , , Datasheet:
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$125.00 – $390.00

  • SizePrice
Clear
Formulation:
Protein G purified
Species Tested:
Rat
Expected Reactivity:
Mouse, Human, Non-human primate
Applications:
WB 1:1000IHC 1:500 Don't see your application?
Host Species:
Sheep Polyclonal
Gene Name:
CALB2
Molecular Weight:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# 303-CAL, RRID:AB_2492050
Antigen/Purification: ExpandCollapse

The antigen is a  purified guinea pig calretinin.

The antibody is protein G purified sheep serum.

Biological Significance: ExpandCollapse

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).

Synonyms: ExpandCollapse
  • 29 kDa calbindin antibody
  • CAB 29 antibody
  • CAB29 antibody
  • CAL 2 antibody
  • CAL2 antibody
  • CALB 2 antibody
  • CALB2 antibody
  • CALB2_HUMAN antibody
  • Calbindin 2 29kDa antibody
  • Calbindin 2 antibody
  • Calbindin D29K antibody
  • Calbindin2 antibody
  • Calretinin antibody
  • CR antibody

Storage

100 µl in 10 mM Tris, 100 mM NaCl. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunohistochemistry

Rat Brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Attanoos, R.L., Dojcinov, S.D., Webb, R. and Gibbs, A.R., 2000. Anti‐mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology, 37(3), pp.224-231. PMID: 10971698

Isaacs, K.R., Winsky, L., Strauss, K.I. and Jacobowitz, D.M., 1995. Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell and tissue research, 280(3), pp.639-651. PMID: 7541720

Lukas, W. and Jones, K.A., 1994. Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neuroscience, 61(2), pp.307-316. PMID: 7969911

Weisenhorn, D.V., Weruaga-Prieto, E. and Celio, M.R., 1996. Calretinin-immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Experimental brain research, 108(1), pp.101-112. PMID: 8721158

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Calretinin Antibody
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.

Click here to view our Western blotting and lysate preparation protocols.
Calretinin Antibody
Immunostaining of rat cerebellum showing fluorescence of calretinin (cat #303-CAL, green, 1:500) containing cells.
Download the protocol used for this image:

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