Calcitonin Gene Related Peptide (CGRP) Antibody

Catalog #: 311-CGRP Categories: , Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Tested:
Human, Rat
Expected Reactivity:
Most mammals
Applications:
WB not recommendedICC 1:50-1:100 Don't see your application?
Species:
Mouse
Gene Name:
CALCA
Cite This Antibody:
PhosphoSolutions Cat# 311-CGRP, RRID:AB_2492054
Antigen/Purification: ExpandCollapse

The antigen is a rat alpha-CGRP.

The antibody is protein G purified.

Biological Significance: ExpandCollapse

Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in immunoneutralization (Wong et al., 1993)

Synonyms: ExpandCollapse
  • Calcitonin/calcitonin related polypeptide alpha antibody
  • Alpha type CGRP antibody
  • Beta type CGRP antibody
  • CALC1 antibody
  • CALC2 antibody
  • CALCA antibody
  • CALCB antibody
  • Calcitonin 1 antibody
  • Calcitonin 2 antibody
  • Calcitonin gene related peptide I antibody
  • Calcitonin gene related peptide II antibody
  • Calcitonin related polypeptide alpha antibody
  • Calcitonin related polypeptide beta antibody
  • CGRP antibody
  • CGRP I antibody
  • CGRP II antibody
  • CGRP1 antibody
  • CGRP2 antibody
  • CT antibody
  • Katacalcin antibody
  • KC antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene related peptide is a potent vasodilator. Nature 313 (5997): 54-6. PMID: 3917554

Edvinsson, L., 2003. New therapeutic target in primary headaches–blocking the CGRP receptor. Expert opinion on therapeutic targets, 7(3), pp.377-383. PMID: 12783573

Wong HC, Tache’ Y, Lloud KCK, Yang H, Sternini C, Holzer P, Walsh JH (1993) Monoclonal antibody to rat alpha-CGRP: production, charactrization, and in vivo immunoneutralization activity. Hybridoma. 12:93-106. PMID: 8095920

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Calcitonin Gene Related Peptide (CGRP) Antibody
Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained and photo was provided courtesy of QBMCellScience.

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