Calbindin Antibody

$109.00$365.00

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Great for identifying specific neuronal cell types in IHC!

Size:
100 µl
Formulation:
Protein G Purified
Specificity:
Rat, Mouse, Human, Bovine
Applications:
WB 1:2,000IF 1:1,000
Species:
Mouse Monoclonal
Gene Name:
CALB1
Molecular Reference:
~ 28 kDa
Cite This Antibody:
PhosphoSolutions Cat# 302-CALB, RRID:AB_2560939
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human calbindin purified from E. coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).

Storage

100 µl in in PBS + 50% glycerol and 5 mM sodium azide.

For long term storage –20° C is recommended. Store at –20° C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

General References

Andressen, C., Blümcke, I., & Celio, M. R. (1993). Calcium-binding proteins: selective markers of nerve cells. Cell and tissue research, 271(2), 181-208.

Kook, S. Y., Jeong, H., Kang, M. J., Park, R., Shin, H. J., Han, S. H., … & Mook-Jung, I. (2014). Crucial role of calbindin-D28k in the pathogenesis of Alzheimer’s disease mouse model. Cell Death & Differentiation. 21, 1575–1587.

Calbindin Antibody
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.
Immunostaining of rat cerebellum showing specific labeling of calbindin (red) in the dendrites of Purkinje cells. Axons are stained green with anti-neurofilament H antibody (cat. # 1451-NFH).

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