Calbindin Antibody

Catalog #: 302-CALB Categories: , , , Datasheet:

$109.00$365.00

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Great for identifying specific neuronal cell types in IHC!

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:1000-1:5000
Species:
Mouse Monoclonal
Gene Name:
CALB1
Molecular Weight:
~ 28 kDa
Cite This Antibody:
PhosphoSolutions Cat# 302-CALB, RRID:AB_2560939
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human calbindin purified from E. coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).

Synonyms: ExpandCollapse
  • avian-type antibody
  • CAB27 antibody
  • CALB 1 antibody
  • CALB antibody
  • CALB1 antibody
  • CALB1_HUMAN antibody
  • Calbindin 1 28kDa antibody
  • Calbindin antibody
  • Calbindin D28 antibody
  • D 28K antibody
  • D-28K antibody
  • D28K antibody
  • OTTHUMP00000166027 antibody
  • OTTHUMP00000225441 antibody
  • RTVL H protein antibody
  • Vitamin D dependent calcium binding protein antibody
  • Vitamin D dependent calcium binding protein avian type antibody
  • Vitamin D-dependent calcium-binding protein antibody

Storage

100 µl in in PBS + 50% glycerol and 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Cerebellum (free float labeled)
Rat Hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Andressen, C., Blümcke, I., & Celio, M. R. (1993). Calcium-binding proteins: selective markers of nerve cells. Cell and tissue research, 271(2), 181-208.

Kook, S. Y., Jeong, H., Kang, M. J., Park, R., Shin, H. J., Han, S. H., … & Mook-Jung, I. (2014). Crucial role of calbindin-D28k in the pathogenesis of Alzheimer’s disease mouse model. Cell Death & Differentiation. 21, 1575–1587.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Calbindin Antibody
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.

Click here to view our Western blotting and lysate preparation protocols.

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