Affinity purified from pooled serum. Learn more.

Beclin-1 (Ser295) Antibody

Catalog #: p117-295 Category: Datasheet:


  • SizePrice

Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine
WB 1:250
Gene Name:
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p117-295, RRID:AB_2492048
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of human beclin-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).

Synonyms: ExpandCollapse
  • PG6 antibody
  • ATG 6 antibody
  • ATG6 antibody
  • ATG6 autophagy related 6 homolog antibody
  • Bcl-2-interacting protein beclin antibody
  • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
  • Beclin 1 autophagy related antibody
  • Beclin-1 antibody
  • Beclin1 antibody
  • BECN 1 antibody
  • Becn1 antibody
  • BECN1_HUMAN antibody
  • Coiled coil myosin like BCL2 interacting protein antibody
  • Coiled-coil myosin-like BCL2-interacting protein antibody
  • GT197 antibody
  • Protein GT197 antibody
  • VPS 30 antibody
  • VPS30 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Song, X., Lee, D.H., Dilly, A.K., Lee, Y.S., Choudry, H.A., Kwon, Y.T., Bartlett, D.L. and Lee, Y.J., 2018. Crosstalk Between Apoptosis and Autophagy Is Regulated by the Arginylated BiP/Beclin-1/p62 Complex. Molecular Cancer Research. Jul;16(7):1077-1091. PMID: 29669822

Weh, K.M., Howell, A.B. and Kresty, L.A., 2016. Expression, modulation, and clinical correlates of the autophagy protein Beclin?1 in esophageal adenocarcinoma. Molecular carcinogenesis, 55(11), pp.1876-1885. PMID: 27696537

Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959. PMID: 23112296

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Beclin-1 ser295 Antibody
Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

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