Affinity purified from pooled serum. Learn more.

Beclin-1 (Ser295) Antibody

Catalog #: p117-295 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine
Applications:
WB 1:250
Species:
Rabbit
Gene Name:
BECN1
Molecular Reference:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p117-295, RRID:AB_2492048
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of human beclin-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Song, X., Lee, D.H., Dilly, A.K., Lee, Y.S., Choudry, H.A., Kwon, Y.T., Bartlett, D.L. and Lee, Y.J., 2018. Crosstalk Between Apoptosis and Autophagy Is Regulated by the Arginylated BiP/Beclin-1/p62 Complex. Molecular Cancer Research. Jul;16(7):1077-1091. PMID: 29669822

Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959. PMID: 23112296

Beclin-1 ser295 Antibody
Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

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