Affinity purified from pooled serum. Learn more.

Beclin-1 (Ser234) Antibody

Catalog #: p117-234 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Canine, Non-human primate, Sheep
Applications:
WB 1:250 Don't see your application?
Species:
Rabbit
Gene Name:
BECN1
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# p117-234, RRID:AB_2492047
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of human beclin-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).

Synonyms: ExpandCollapse
  • PG6 antibody
  • ATG 6 antibody
  • ATG6 antibody
  • ATG6 autophagy related 6 homolog antibody
  • Bcl-2-interacting protein beclin antibody
  • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
  • Beclin 1 autophagy related antibody
  • Beclin-1 antibody
  • Beclin1 antibody
  • BECN 1 antibody
  • Becn1 antibody
  • BECN1_HUMAN antibody
  • Coiled coil myosin like BCL2 interacting protein antibody
  • Coiled-coil myosin-like BCL2-interacting protein antibody
  • GT197 antibody
  • Protein GT197 antibody
  • VPS 30 antibody
  • VPS30 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western blot: Human
PMID Dilution Publication
27696537 1:250 Weh, K.M., et al. 2016 Expression, modulation, and clinical correlates of the autophagy protein Beclin‐1 in esophageal adenocarcinoma. Molecular carcinogenesis, 55(11), pp.1876-1885.
23112296 not listed Wang, R.C., et al. 2012 Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.


Western blot: Mouse
PMID Dilution Publication
29669822 not listed Song, X., et al. 2018 Crosstalk Between Apoptosis and Autophagy Is Regulated by the Arginylated BiP/Beclin-1/p62 Complex. Molecular Cancer Research. Jul;16(7):1077-1091.


Western blot: Rat
PMID Dilution Publication
23112296 not listed Wang, R.C., et al. 2012 Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Beclin-1 ser234 Antibody
Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.

Click here to view our Western blotting and lysate preparation protocols.

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