Affinity purified from pooled serum. Learn more.

ATRIP (Ser239) Antibody

We are the original manufacturer of our ATRIP (Ser239) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p116-239 Categories: , , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Expected Reactivity:
Human, Bovine, Non-human primate, Sheep
WB 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~86 kDa
Cite This Antibody:
PhosphoSolutions Cat# p116-239, RRID:AB_2492046
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser239 of human ATRIP.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser239 (Venere et al, 2007).

Synonyms: ExpandCollapse
  • AGS1 antibody
  • ATIP antibody
  • ATM and Rad3 related interacting protein antibody
  • ATM and Rad3-related-interacting protein antibody
  • ATR interacting protein antibody
  • ATR-interacting protein antibody
  • Atrip antibody
  • ATRIP_HUMAN antibody
  • DKFZp762J2115 antibody
  • FLJ12343 antibody
  • MGC20625 antibody
  • MGC21482 antibody
  • MGC26740 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Cortez D, Guntuku S, Qin J, Elledge SJ (2001) ATR and ATRIP: partners in checkpoint signaling. Science 294(5547):1713-6. PMID: 11721054

Ball HL, Ehrhardt MR, Mordes DA, Glick GG, Chazin WJ, Cortex D. (2007) Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol Cel Biol. 27(9):3367-77. PMID: 17339343

Venere M, Synder A, Zgheib, and Halazonetis T. (2007) Phosphorylation of ATR-intereacting Protien on Ser239 Mediates an Interaction with Breast-Ovarian Cancer Susceptibility 1 and Checkpoint Function. Cancer Res 67(13): 6100-5. PMID: 17616665

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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