Affinity purified from pooled serum. Learn more.

ATF2 (Thr52) Antibody

Catalog #: p115-52 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Human, Non-human primate
Applications:
WB 1:250
Species:
Rabbit
Gene Name:
ATF2
Molecular Reference:
~72 kDa
Cite This Antibody:
PhosphoSolutions Cat# p115-52, RRID:AB_2492045
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr52 of human ATF2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKC? regulates this translocation (Lau et al., 2012).

Synonyms: ExpandCollapse
  • Activating transcription factor 2 antibody
  • Activating transcription factor 2 splice variant ATF2 var2 antibody
  • ATF 2 antibody
  • Atf-2 antibody
  • Atf2 antibody
  • ATF2 protein antibody
  • ATF2_HUMAN antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP response element binding protein CRE BP1 antibody
  • cAMP response element-binding protein CRE-BP1 antibody
  • cAMP responsive element binding protein 2, formerly antibody
  • cAMP-dependent transcription factor ATF-2 antibody
  • cAMP-responsive element-binding protein 2 antibody
  • CRE BP1 antibody
  • CRE-BP antibody
  • CREB 2 antibody
  • CREB-2 antibody
  • CREB2 antibody
  • CREBP1 antibody
  • Cyclic AMP dependent transcription factor ATF 2 antibody
  • Cyclic AMP-dependent transcription factor ATF-2 antibody
  • Cyclic AMP-responsive element-binding protein 2 antibody
  • D130078H02Rik antibody
  • D18875 antibody
  • HB 16 antibody
  • HB16 antibody
  • Histone acetyltransferase ATF2 antibody
  • MGC105211 antibody
  • MGC105222 antibody
  • MGC111558 antibody
  • MGC142504 antibody
  • mXBP antibody
  • MXBP protein antibody
  • Tg(Gzma-Klra1)7Wum antibody
  • TREB 7 antibody
  • TREB7 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Kumar, V., Weng, Y.C., Wu, Y.C., Huang, Y.T. and Chou, W.H., 2018. PKC? phosphorylation regulates the mitochondrial translocation of ATF2 in ischemia-induced neurodegeneration. BMC neuroscience19(1), p.76. PMID: 30497386

Lau E, Sedy J, Sander C, Shaw MA, Feng Y, Scortegagna M, Claps G, Robinson S, Cheng P, Srivas R, Soonthornvacharin S, Ideker T, Bosenberg M, Gonzalez R, Robinson W, Chanda SK, Ware C, Dummer R, Hoon D, Kirkwood JM, Ronai ZA. (2015). Transcriptional repression of IFN?1 by ATF2 confers melanoma resistance to therapy. Oncogene. March 2. PMID: 25728676

Varsano, T., Lau, E., Feng, Y., Garrido, M., Milan, L., Heynen-Genel, S., Hassig, C.A. & Ze’ev, A. R. (2013). Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2. Clinical Cancer Research, 19(10), 2710-2722. PMID: 23589174

Lau E, Kluger H, Varsano T, Lee K, Scheffler I, Rimm D, Ideker T, and Ronai Z. (2012) PKC Promote Oncogenic Functions of ATF2 in the Nucleus while Blocking Its Apoptotic Function at Mitochondria. Cell 148, 543-555. PMID: 22304920

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ATF2 thr52 Antibody
Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~ 72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Gö6850. Image courtesy of Eric Lau and Ze’ev Ronai.

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