Affinity purified from pooled serum. Learn more.

ATF2 (Thr52) Antibody

Catalog #: p115-52 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, Non-human primate
Applications:
WB 1:250
Species:
Rabbit
Gene Name:
ATF2
Molecular Reference:
~72 kDa
Cite This Antibody:
PhosphoSolutions Cat# p115-52, RRID:AB_2492045
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr52 of human ATF2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKCε regulates this translocation (Lau et al., 2012).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Kumar, V., Weng, Y.C., Wu, Y.C., Huang, Y.T. and Chou, W.H., 2018. PKCε phosphorylation regulates the mitochondrial translocation of ATF2 in ischemia-induced neurodegeneration. BMC neuroscience19(1), p.76. PMID: 30497386

Lau E, Sedy J, Sander C, Shaw MA, Feng Y, Scortegagna M, Claps G, Robinson S, Cheng P, Srivas R, Soonthornvacharin S, Ideker T, Bosenberg M, Gonzalez R, Robinson W, Chanda SK, Ware C, Dummer R, Hoon D, Kirkwood JM, Ronai ZA. (2015). Transcriptional repression of IFNβ1 by ATF2 confers melanoma resistance to therapy. Oncogene. March 2. PMID: 25728676

Varsano, T., Lau, E., Feng, Y., Garrido, M., Milan, L., Heynen-Genel, S., Hassig, C.A. & Ze’ev, A. R. (2013). Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2. Clinical Cancer Research, 19(10), 2710-2722. PMID: 23589174

Lau E, Kluger H, Varsano T, Lee K, Scheffler I, Rimm D, Ideker T, and Ronai Z. (2012) PKC Promote Oncogenic Functions of ATF2 in the Nucleus while Blocking Its Apoptotic Function at Mitochondria. Cell 148, 543-555. PMID: 22304920

ATF2 thr52 Antibody
Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~ 72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Gö6850. Image courtesy of Eric Lau and Ze’ev Ronai.

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