ATF2 (Ser490,498) Antibody

Catalog #: p115-4908 Category: Datasheet:

$310.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Specificity:
Rat, Human
Applications:
WB 1:1000IHC 1:1000
Species:
Rabbit
Gene Name:
ATF2
Molecular Reference:
~74 kDa
Cite This Antibody:
PhosphoSolutions Cat# p115-4908, RRID:AB_2492044
Antigen/Purification: ExpandCollapse

The antigen is a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser490,498 of human ATF2.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003; Morton et al., 2004). Stress induced ATFdependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000); Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Muñoz, Denise P., Misako Kawahara, and Steven M. Yannone. “An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells.” Nucleic acids research 41.5 (2013): 2894-2906. PMID: 23325849

Bhoumik A, Takahashi S, Breitweiser W, Shiloh Y, Jones N, Ronai Z (2005) ATM-dependent phosphorylation of ATF2 is required for DNA damage response. Mol Cell May 27; 18(5):577-87. PMID: 15916964

 

 

ATF2 ser 490,498 Antibody
Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

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