Arginase I/Liver Arginase (ARG1) Antibody

Catalog #: 146-ARG1 Category: Datasheet:

$109.00$365.00

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Chicken polyclonal antibody

Formulation:
Totaly IgY fraction
Specificity:
Guinea pig, Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:500 (Poljakovic M et al., 2007)
Species:
Chicken Polyclonal
Gene Name:
ARG1
Molecular Reference:
~35-38 kDa
Cite This Antibody:
PhosphoSolutions Cat# 146-ARG1, RRID:AB_2732850
Antigen/Purification: ExpandCollapse

The antigen is full-length recombinant rat liver arginase I purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996).  Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References:

Poljakovic M, Porter DW, Millecchia L, Kepka-Lenhart D, Beighley C, Wolfarth MG, Castranova V, Morris SM. Cell- and Isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. J Toxicol Environ Health A 15;70(2):118-127 (2007).

Morris SM, Kepka-Lenhart D, Chen LC. Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. Am J Physiol 275(5 Pt 1):E740-7 (1998).

 

Western blot of Arginase I/Liver Arginase ARG1 Antibody
Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.

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