Arginase I/Liver Arginase (ARG1) Antibody

Catalog #: 146-ARG1 Category: Datasheet:

$109.00$365.00

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Chicken polyclonal antibody

Formulation:
Total IgY fraction
Species Reactivity:
Guinea pig, Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:500
Species:
Chicken Polyclonal
Gene Name:
ARG1
Molecular Weight:
~35-38 kDa
Cite This Antibody:
PhosphoSolutions Cat# 146-ARG1, RRID:AB_2732850
Antigen/Purification: ExpandCollapse

The antigen is full-length recombinant rat liver arginase I purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996).  Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

Synonyms: ExpandCollapse
  • A I antibody
  • Al antibody
  • ARG 1 antibody
  • arg1 antibody
  • ARGI1_HUMAN antibody
  • Arginase 1 antibody
  • Arginase liver antibody
  • Arginase type I antibody
  • Arginase, liver antibody
  • Arginase-1 antibody
  • Arginase1 antibody
  • Liver type arginase antibody
  • Liver-type arginase antibody
  • Type I arginase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Poljakovic M, Porter DW, Millecchia L, Kepka-Lenhart D, Beighley C, Wolfarth MG, Castranova V, Morris SM. Cell- and Isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. J Toxicol Environ Health A 15;70(2):118-127 (2007). PMID: 17365572

Morris SM, Kepka-Lenhart D, Chen LC. Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. Am J Physiol 275(5 Pt 1):E740-7 (1998). PMID: 9814991

 

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of Arginase I/Liver Arginase ARG1 Antibody
Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.
Immunofluorescent image of mouse retina specifically labeling arginase (catalog #145-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. Frozen sections of mouse retina were initially fixed with 4% PFA for 2.5 hours, and then froze before sectioning and mounting onto glass slides. The tissues were blocked with 2% donkey serum, 0.3% Triton-X in PBS for 30 minutes before incubating with primary antibody overnight at room temperature. The anti-arginase antibody was diluted in the blocking buffer. An Alexa-488 donkey anti-chicken secondary antibody was used at 1:200 at room temperature for 1 hour. The tissue sections were washed with PBS between each of the blocking and incubation steps. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

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