Arginase I/Liver Arginase (ARG1) Antibody

Our chicken polyclonal Arginase I (ARG1) antibody is total IgY fraction. It is excellent for use in WB and IHC.

Catalog #: 146-ARG1 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Total IgY fraction
Species Tested:
Guinea pig, Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:500 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
ARG1
Molecular Weight:
~35-38 kDa
Cite This Antibody:
PhosphoSolutions Cat# 146-ARG1, RRID:AB_2732850
Antigen/Purification: ExpandCollapse

The antigen is full-length recombinant rat liver arginase I purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996).  Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

Synonyms: ExpandCollapse
  • A I antibody
  • Al antibody
  • ARG 1 antibody
  • arg1 antibody
  • ARGI1_HUMAN antibody
  • Arginase 1 antibody
  • Arginase liver antibody
  • Arginase type I antibody
  • Arginase, liver antibody
  • Arginase-1 antibody
  • Arginase1 antibody
  • Liver type arginase antibody
  • Liver-type arginase antibody
  • Type I arginase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat
Western Blot: Mouse


Immunohistochemistry: Rat
PMID Dilution Publication
17365572 1:500 Poljakovic, M., et al. 2007 Cell-and isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. Journal of Toxicology and Environmental Health, Part A, 70(2), pp.118-127.


Western Blot: Mouse
PMID Dilution Publication
9814991 not listed Morris, S., et al. 1998 Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. American Journal of Physiology-Endocrinology And Metabolism, 275(5), pp.E740-E747.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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