Affinity purified from pooled serum. Learn more.

Aquaporin 2 (Ser269) Antibody

Catalog #: p112-269 Category: Datasheet:


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Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Bovine, Canine
WB 1:1000
Gene Name:
Molecular Reference:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p112-269, RRID:AB_2492043
Antigen/Purification: ExpandCollapse

The antibody is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of rat aquaporin 2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).

Synonyms: ExpandCollapse
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  • AQP-2 antibody
  • AQP-CD antibody
  • AQP2 antibody
  • AQP2_HUMAN antibody
  • AQPCD antibody
  • Aquaporin 2 collecting duct antibody
  • Aquaporin CD antibody
  • Aquaporin-2 antibody
  • Aquaporin-CD antibody
  • Aquaporin2 antibody
  • Aquaporine 2 antibody
  • Collecting duct water channel protein antibody
  • MGC34501 antibody
  • Water channel aquaporin 2 antibody
  • Water channel protein for renal collecting duct antibody
  • WCH CD antibody
  • WCH-CD antibody
  • WCHCD antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Lei, L., Huang, M., Su, L., Xie, D., Mamuya, F.A., Ham, O., Tsuji, K., P?unescu, T.G., Yang, B. and Lu, H.A.J., 2017. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391. PMID: 29046300

Yui, N., Ando, F., Sasaki, S. and Uchida, S., 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. 2017. Aug 26;490(3):1039-1044. PMID: 28668390

Yui, N., Sasaki, S. and Uchida, S., 2016. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and Biophysical Research Communications.  2017 Jan 22;482(4):524-529. PMID:  27889609

Gao, Y., Stuart, D., Pollock, J.S., Takahashi, T. and Kohan, D.E., 2016. Collecting duct-specific knockout of nitric oxide synthase 3 impairs water excretion in a sex-dependent manner. American Journal of Physiology-Renal Physiology, pp.ajprenal-00494. PMID: 27707708

Speed, J. S., & Hyndman, K. A. (2016). In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6. PMID: 27185292

Choi, H. J., Jung, H. J., & Kwon, T. H. (2015). Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. American Journal of Physiology-Renal Physiology, 308(7), F737-F748. PMID: 25651562

Mamenko M, Dhande I, Tomilin V, Zaika O, Boukelmoune N, Zhu Y, Gonzales-Garay ML, Pochynyuk O, & Doris, P. (2015) Defective Store-Operated Calcium Entry Causes Partial Nephrogenic Diabetes Insipidus. J. Am. Soc. Nephrol. Nov 16. PMID: 26574044

Rice, W. L., Li, W., Mamuya, F., McKee, M., P?unescu, T. G., & Lu, H. A. J. (2015). Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. PMID: 26147297

Hoffert, J. D., Pisitkun, T., Saeed, F., Wilson, J. L., & Knepper, M. A. (2014). Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421. PMID: 24259510

Bogum, J., Faust, D., Zühlke, K., Eichhorst, J., Moutty, M. C., Furkert, J., Eldahshan A, Neuenschwander M, von Kries JP, Wiesner B, & Trimpert, C. (2013). Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758. PMID: 23559583

Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. PMID: 18606813



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Aquaporin 2 ser269 Antibody
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

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