Affinity purified from pooled serum. Learn more.

Aquaporin 2 (Ser269) Antibody

Catalog #: p112-269 Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Species Reactivity:
Bovine, Canine
Applications:
WB 1:1000IHC 1:600-1:5000IP 3 ulICC 1:500
Species:
Rabbit
Gene Name:
AQP2
Molecular Weight:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p112-269, RRID:AB_2492043
Antigen/Purification: ExpandCollapse

The antibody is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of rat aquaporin 2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).

Synonyms: ExpandCollapse
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  • AQP-2 antibody
  • AQP-CD antibody
  • AQP2 antibody
  • AQP2_HUMAN antibody
  • AQPCD antibody
  • Aquaporin 2 collecting duct antibody
  • Aquaporin CD antibody
  • Aquaporin-2 antibody
  • Aquaporin-CD antibody
  • Aquaporin2 antibody
  • Aquaporine 2 antibody
  • Collecting duct water channel protein antibody
  • MGC34501 antibody
  • Water channel aquaporin 2 antibody
  • Water channel protein for renal collecting duct antibody
  • WCH CD antibody
  • WCH-CD antibody
  • WCHCD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Kidney

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Mouse | Rat
Western Blot: Mouse | Rat


Immunocytochemistry: Rat
PMID Dilution Publication
28668390 1:500 Yui, N., et al. 2017 Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.


Immunohistochemistry: Mouse
PMID Dilution Publication
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.


Immunohistochemistry: Rat
PMID Dilution Publication
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
23559583 1:600 Bogum, J., et al. 2013 Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.


Immunoprecipitation: Mouse
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.


Immunoprecipitation: Rat
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.


Western Blot: Mouse
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609 1:500-1:1000 Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27707708 1:1000 Gao, Y., et al. 2016 Collecting duct-specific knockout of nitric oxide synthase 3 impairs water excretion in a sex-dependent manner. American Journal of Physiology-Renal Physiology, pp.ajprenal-00494.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.


Western Blot: Rat
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390 1:500 Yui, N., et al. 2017 Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609 1:500-1:1000 Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994 not listed Al-Butaineh, M.M, et al. 2016 Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292 not listed Speed, J.S, et al. 2016 In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26574044 1:5000 Mamenko, M., et al. 2016 Defective store-operated calcium entry causes partial nephrogenic diabetes insipidus. Journal of the American Society of Nephrology, 27(7), pp.2035-2048.
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
25651562 1:1000 Choi,H. J., et al. 2015 Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48.
24259510 1:1000 Hoffert, J.D., et al. 2014 Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421.
23559583 1:600 Bogum, J., et al. 2013 Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Aquaporin 2 ser269 Antibody
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

Click here to view our Western blotting and lysate preparation protocols.

You may also like…