Affinity purified from pooled serum. Learn more.

Aquaporin 2 (Ser264) Antibody

Catalog #: p112-264 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
AQP2
Molecular Reference:
~ 29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p112-264, RRID:AB_2492042
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser264 of rat aquaporin 2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-264 has been identified as a novel phosphorylation site on AQP2 and shown to be regulated by vasopressin thus implicating this site as important in AQP2 trafficking and subcellular distribution (Fenton RA et al., 2008).

Synonyms: ExpandCollapse
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  • AQP-2 antibody
  • AQP-CD antibody
  • AQP2 antibody
  • AQP2_HUMAN antibody
  • AQPCD antibody
  • Aquaporin 2 collecting duct antibody
  • Aquaporin CD antibody
  • Aquaporin-2 antibody
  • Aquaporin-CD antibody
  • Aquaporin2 antibody
  • Aquaporine 2 antibody
  • Collecting duct water channel protein antibody
  • MGC34501 antibody
  • Water channel aquaporin 2 antibody
  • Water channel protein for renal collecting duct antibody
  • WCH CD antibody
  • WCH-CD antibody
  • WCHCD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Lei, L., Huang, M., Su, L., Xie, D., Mamuya, F.A., Ham, O., Tsuji, K., Păunescu, T.G., Yang, B. and Lu, H.A.J., 2017. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391. PMID: 29046300

Al-Bataineh, M. M., Li, H., Ohmi, K., Gong, F., Marciszyn, A., Naveed, S., Zhu, X., Neumann, D., Wu, Q., Cheng, L., & Fenton, R. A. (2016). Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.  PMID: 27534994

Speed, J. S., & Hyndman, K. A. (2016). In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6. PMID: 27185292

Choi, H. J., Jung, H. J., & Kwon, T. H. (2015). Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. American Journal of Physiology-Renal Physiology, 308(7), F737-F748. PMID: 25651562

Rice, W. L., Li, W., Mamuya, F., McKee, M., Păunescu, T. G., & Lu, H. A. J. (2015). Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. PMID: 26147297

Hoffert, J. D., Pisitkun, T., Saeed, F., Wilson, J. L., & Knepper, M. A. (2014). Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421. PMID: 24259510

Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27 PMID: 18606813

Fenton, R. A., Moeller, H. B., Hoffert, J. D., Yu, M. J., Nielsen, S., & Knepper, M. A. (2008). Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin. Proceedings of the National Academy of Sciences, 105(8), 3134-3139. PMID: 18287043

Hoffert JD, Nielsen J,, Yu MJ, Pisitikun T, Schleicher SM, Nielsen S, Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. PMID: 16985212

 

 

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Aquaporin 2 ser264 Antibody
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser264 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

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