Affinity purified from pooled serum. Learn more.

Aquaporin 2 (Ser264) Antibody

Catalog #: p112-264 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Species Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate
Applications:
WB 1:1000IHC 1:100
Species:
Rabbit
Gene Name:
AQP2
Molecular Weight:
~ 29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p112-264, RRID:AB_2492042
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser264 of rat aquaporin 2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-264 has been identified as a novel phosphorylation site on AQP2 and shown to be regulated by vasopressin thus implicating this site as important in AQP2 trafficking and subcellular distribution (Fenton RA et al., 2008).

Synonyms: ExpandCollapse
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  • AQP-2 antibody
  • AQP-CD antibody
  • AQP2 antibody
  • AQP2_HUMAN antibody
  • AQPCD antibody
  • Aquaporin 2 collecting duct antibody
  • Aquaporin CD antibody
  • Aquaporin-2 antibody
  • Aquaporin-CD antibody
  • Aquaporin2 antibody
  • Aquaporine 2 antibody
  • Collecting duct water channel protein antibody
  • MGC34501 antibody
  • Water channel aquaporin 2 antibody
  • Water channel protein for renal collecting duct antibody
  • WCH CD antibody
  • WCH-CD antibody
  • WCHCD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Kidney

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Mouse | Rat


Immunohistochemistry: Mouse
PMID Dilution Publication
18606813 1:100 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.


Immunohistochemistry: Rat
PMID Dilution Publication
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
18606813 1:100 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
18287043 not listed Fenton, R.A., et al. 2007 Acute regulation of aquaporin-2 phosphorylation at serine-264 by vasopressin. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3134-9.


Western Blot: Mouse
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.


Western Blot: Rat
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
27534994 not listed Al-Butaineh, M.M, et al. 2016 Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292 not listed Speed, J.S, et al. 2016 In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
25651562 1:1000 Choi, H.J., et al. 2015 Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48.
24259510 1:1000 Hoffert, J.D., et al. 2014 Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212 1:2000 Hoffert, J.D., et al. 2007 Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Aquaporin 2 ser264 Antibody
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser264 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

Click here to view our Western blotting and lysate preparation protocols.

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