Affinity purified from pooled serum. Learn more.

Aquaporin 2 (Ser261) Antibody

Catalog #: p112-261 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate
Applications:
WB 1:1000IHC 1:2000IP 3 ulICC 1:500-1:300
Species:
Rabbit
Gene Name:
AQP2
Molecular Weight:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p112-261, RRID:AB_2492041
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007)

Synonyms: ExpandCollapse
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  • AQP-2 antibody
  • AQP-CD antibody
  • AQP2 antibody
  • AQP2_HUMAN antibody
  • AQPCD antibody
  • Aquaporin 2 collecting duct antibody
  • Aquaporin CD antibody
  • Aquaporin-2 antibody
  • Aquaporin-CD antibody
  • Aquaporin2 antibody
  • Aquaporine 2 antibody
  • Collecting duct water channel protein antibody
  • MGC34501 antibody
  • Water channel aquaporin 2 antibody
  • Water channel protein for renal collecting duct antibody
  • WCH CD antibody
  • WCH-CD antibody
  • WCHCD antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Kidney

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Mouse | Rat
Western Blot: Mouse | Rat


Immunocytochemistry: Rat
PMID Dilution Publication
28668390 1:500 Yui, N., et al. 2017 Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.
27760768 1:3000 Mamuya, F.A., et al. 2016 ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336.


Immunohistochemistry: Mouse
PMID Dilution Publication
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212 1:2000 Hoffert, J.D., et al. 2007 Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.


Immunohistochemistry: Rat
PMID Dilution Publication
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212 1:2000 Hoffert, J.D., et al. 2007 Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.


Immunoprecipitation: Mouse
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.


Immunoprecipitation: Rat
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.


Western Blot: Mouse
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609 1:500-1:1000 Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27760768 1:3000 Mamuya, F.A., et al. 2016 ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212 1:2000 Hoffert, J.D., et al. 2007 Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.


Western Blot: Rat
PMID Dilution Publication
29046300 1:3000 Lei, L., et al. 2018 Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390 1:500 Yui, N., et al. 2017 Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609 1:500-1:1000 Yui, N., et al. 2017 Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994 not listed Al-Butaineh, M.M, et al. 2016 Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292 not listed Speed, J.S, et al. 2016 In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26147297 not listed Rice, W.L., et al. 2015 Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
18606813 1:2000 Hoffert, J.D., et al. 2008 Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212 1:2000 Hoffert, J.D., et al. 2007 Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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