Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Xenopus, Chicken, Non-human primate, Zebra fish, Guinea Pig
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~28 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1860-244, RRID:AB_2492234
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser244 of mouse rpS6.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5′-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser235, Ser236, Ser240, and Ser244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).