Affinity purified from pooled serum. Learn more.

Ribosomal Protein S6 (Ser244) Antibody

Catalog #: p1860-244 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Xenopus, Chicken, Non-human primate, Zebra fish, Guinea Pig
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
RPS6
Molecular Reference:
~28 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1860-244, RRID:AB_2492234
Antigen/Purification: ExpandCollapse

The antigen is  a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser244 of mouse rpS6.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5?-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser235, Ser236, Ser240, and Ser244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-?), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).

Synonyms: ExpandCollapse

• 40S ribosomal protein S6 antibody
• Air8 antibody
• NP33 antibody
• Phosphoprotein NP33 antibody
• Pp30 antibody
• Ribosomal protein S6 antibody
• RP S6 antibody
• rps6 antibody
• RPS6 antibody
• RS6 antibody
• RS6_HUMAN antibody
• S6 antibody
• S6 Ribosomal Protein antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Chen W and Dittmer D (2011) Ribosomal protein S6 (RPS6) interacts with the latency-associated nuclear antigen (LANA) of Kaposi sarcoma associated herpesvirus (KSHV). J. Virol. 18 9495-9505

Fumagalli S. and Thomas, G. (2000). S6 phosphorylation and signal transduction. In Translational control of gene expression (eds. N. Sonenberg et al.), pp. 695-717. Cold

Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Hutchinson JA, Shanware NP, Chang H, Tibbetts RS. (2011) Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1. J Biol Chem 10:8688-96

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Ribosomal Protein S6 Ser244 Antibody
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~28 kDa rpS6 phosphorylated at Ser244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

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