Affinity purified from pooled serum. Learn more.

Ribosomal Protein S6 (Ser244) Antibody

We are the original manufacturer of our Ribosomal Protein S6 (Ser244) rabbit polyclonal phosphospecific antibody. It is affinity purified from pooled serum, and is excellent in WB.

Catalog #: p1860-244 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Expected Reactivity:
Chicken, Guinea Pig, Non-human primate, Rat, Xenopus, Zebra fish
Applications:
WB 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
RPS6
Molecular Weight:
~28 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1860-244, RRID:AB_2492234
Antigen/Purification: ExpandCollapse

The antigen is  a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser244 of mouse rpS6.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5′-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser235, Ser236, Ser240, and Ser244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).

Synonyms: ExpandCollapse

• 40S ribosomal protein S6 antibody
• Air8 antibody
• NP33 antibody
• Phosphoprotein NP33 antibody
• Pp30 antibody
• Ribosomal protein S6 antibody
• RP S6 antibody
• rps6 antibody
• RPS6 antibody
• RS6 antibody
• RS6_HUMAN antibody
• S6 antibody
• S6 Ribosomal Protein antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Chen, W. and Dittmer, D.P., 2011. Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi’s sarcoma-associated herpesvirus. Journal of Virology, 85(18), pp.9495-9505. PMID: 21734034

Fumagalli, S. and Thomas, G., 2000. 23-S6 Phosphorylation and Signal Transduction. Cold Spring Harbor Monograph Archive , 39, pp.695-717.

Hutchinson, J.A., Shanware, N.P., Chang, H. and Tibbetts, R.S., 2011. Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1. Journal of Biological Chemistry, 286(10), pp.8688-8696. PMID: 21233202

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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