Affinity purified from pooled serum. Learn more.

Phospholipase C β2 (PLC β2) Antibody

Catalog #: 1640-PLC Categories: , Datasheet:

$109.00$365.00

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Excellent human taste cell marker!

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Non-human primate
Applications:
IF 1:1000IHC 1:1000
Species:
Rabbit Polyclonal
Gene Name:
PLCB2
Molecular Weight:
~135 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1640-PLC, RRID:AB_2801663
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of human PLC β2.

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Phospholipases are quite common enzymes that are present in a broad range of organisms, including bacteria, yeast, plants, animals, and viruses. Phospholipase C (PLC) constitutes a class of enzymes that cleave phospholipids on the diacylglycerol (DAG) side of the phosphodiester bond (Cocco et al., 2015). A growing body of evidence supports the role of phospholipase C (PLC) in the invasion and metastasis of different tumors, including breast cancer (Bertagnolo et al., 2006). PLC-β2 has been shown to be required for sweet, umami and bitter taste perception in mammals (Zhang et al., 2003). Data also suggests that PLC-β2 serves an unappreciated role assembling components of the p38MAPK signaling module (Barr et al., 2002).

Synonyms: ExpandCollapse
  • 1-phosphatidylinositol-4 antibody
  • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 antibody
  • 5-bisphosphate phosphodiesterase beta-2 antibody
  • FLJ38135 antibody
  • Phosphoinositide phospholipase C-beta-2 antibody
  • Phospholipase C-beta-2 antibody
  • PLC-beta-2 antibody
  • Plcb2 antibody
  • PLCB2_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C.

General References

Cocco L, Follo MY, Manzoli L, Suh PG. (2015) Phosphoinositide-specific phospholipase C in health and disease. J Lipid Res. (10):1853-60.

Bertagnolo V, Benedusi M, Querzoli P, Pedriali M, Magri E, Brugnoli F, Capitani S. (2006) PLC-beta2 is highly expressed in breast cancer and is associated with a poor outcome: a study on tissue microarrays. Int J Oncol. (4):863-7

Zhang, Y., Hoon, M. A., Chandrashekar, J., Mueller, K. L., Cook, B., Wu, D., Ryba, N. J. P. (2003) Coding of Sweet, Bitter, and Umami Tastes. Cell, 112(3), 293–301.

Barr AJ, Marjoram R,Xu J, Snyderman R. (2002) Phospholipase C-2 interacts with mitogen-activated protein kinase kinase 3. Biochem Biophys Res Commun. (1):647-52.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Immunostaining of human larynx taste bud showing specific labeling of PLC β2 (red) and GNAT3 (green). Photo courtesy of Mei Li, University of Colorado-Anschutz MC.

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