MARCKS Antibody

Excellent human synapse marker!

Catalog #: 1107-MARCKS Categories: , , Datasheet:


  • SizePrice
Total IgY fraction
Species Tested:
Expected Reactivity:
Non-Human Primate
WB 1:10000IHC 1:500-1:1000ICC 1:500-1:5000 Don't see your application?
Host Species:
Chicken polyclonal
Gene Name:
Molecular Weight:
~ 80 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1107-MARCKS: RRID:AB_2751023
Antigen/Purification: ExpandCollapse

The antigen is recombinant human MARCKS expressed in and purified from E. coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992).  Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

Synonyms: ExpandCollapse

• 80 kDa protein antibody
• 80K L antibody
• 80K L protein antibody
• 80K-L protein antibody
• 80KL antibody
• 81 kDa protein, light chain antibody
• light chain antibody
• MACS antibody
• MARCKS antibody
• MARCS antibody
• MARCS_HUMAN antibody
• MGC52672 antibody
• myristoylated alanine rich C kinase substrate antibody
• Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
• Myristoylated alanine rich protein kinase C substrate antibody
• Myristoylated alanine-rich C-kinase substrate antibody
• Phosphomyristin antibody
• PKCSL antibody
• PRKCSL antibody
• protein kinase C substrate 80 kDa protein light chain antibody
• Protein kinase C substrate antibody


100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols


HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. Journal of Neuroscience 10:1683-1698. PMID: 2332803

Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992)  MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. PMID: 1560845

Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995)  MARCKS deficiency in mice leads to abnormal brain development and perinatal death. PNAS 92; 944-948. PMID: 7862670

Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. Journal of Biological Chemistry  269:9361-9367. PMID: 8132675

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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