MARCKS Antibody

Catalog #: 1107-MARCKS Categories: , Datasheet:

$109.00$365.00

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Excellent human synapse marker!

Formulation:
Total IgY fraction
Specificity:
Human, Non-Human Primate
Applications:
WB 1:10,000IF 1:1,000
Species:
Chicken polyclonal
Gene Name:
MARCKS
Molecular Reference:
~ 80 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1107-MARCKS: RRID:AB_2751023
Antigen/Purification: ExpandCollapse

The antigen is recombinant human MARCKS expressed in and purified from E. coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992).  Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

Storage

Shipped on blue ice. Store at –20oC in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

General References:

Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698.

Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992)  MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622

Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995)  MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948.

Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated

alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem  269:9361-9367.

 

Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.
Immunostaining of HeLa cells showing specific labeling of the MARCKS protein in red and B-tubulin in green.