MARCKS Antibody

Catalog #: 1107-MARCKS Categories: , Datasheet:

$109.00$365.00

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Excellent human synapse marker!

Formulation:
Total IgY fraction
Species Tested:
Human
Species Reactivity:
Non-Human Primate
Applications:
WB 1:10000IHC 1:500-1:1000ICC 1:500-1:5000
Species:
Chicken polyclonal
Gene Name:
MARCKS
Molecular Weight:
~ 80 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1107-MARCKS: RRID:AB_2751023
Antigen/Purification: ExpandCollapse

The antigen is recombinant human MARCKS expressed in and purified from E. coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992).  Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

Synonyms: ExpandCollapse

• 80 kDa protein antibody
• 80K L antibody
• 80K L protein antibody
• 80K-L protein antibody
• 80KL antibody
• 81 kDa protein, light chain antibody
• light chain antibody
• MACS antibody
• MARCKS antibody
• MARCS antibody
• MARCS_HUMAN antibody
• MGC52672 antibody
• myristoylated alanine rich C kinase substrate antibody
• Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
• Myristoylated alanine rich protein kinase C substrate antibody
• Myristoylated alanine-rich C-kinase substrate antibody
• Phosphomyristin antibody
• PKCSL antibody
• PRKCSL antibody
• protein kinase C substrate 80 kDa protein light chain antibody
• Protein kinase C substrate antibody

Storage

100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References:

Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698. PMID: 2332803

Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992)  MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. PMID: 1560845

Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995)  MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948. PMID: 7862670

Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem  269:9361-9367. PMID: 8132675

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunolabeling of HeLa cells showing specific labeling of the MARCKs protein (anti-MARCKS antibody, cat #1107-MARCKS, red, 1:5000) and β-tubulin protein (green). Anti-MARCKS binds MARCKs protein expressed in the plasma membrane and cytoplasm. The blue is Hoechst staining the nuclear DNA.

Download the protocol used for this image: