Ki-67 Antibody

Catalog #: 1006-Ki67 Categories: , Datasheet:


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Excellent marker for cell growth!

Totaly IgY fraction
human, mouse, rat
WB 1:5,000IF 1:1000IHC 1:1000
Chicken Polyclonal
Gene Name:
Molecular Reference:
Cite This Antibody:
PhosphoSolutions Cat# 1006-Ki67: RRID:AB_2737605
Antigen/Purification: ExpandCollapse

The antigen is recombinant human Ki-67 purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Ki-67 is a nuclear protein that is  considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000).  Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).


Liquid. Contains 10 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year.  Avoid freeze/thaw cycles.

General References:

 Scholzen T, Gerdes J. (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol. Mar;182(3):311-22

Yerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA (2010). Ki67 in breast cancer: prognostic and predictive potential. The Lancet. Oncology. 11 (2): 174–83.

Folescu R, Levai CM, Grigoraş ML, Arghirescu TS, Talpoş IC, Gîndac CM, Zamfir CL, Poroch V, Anghel MD. (2018) Expression and significance of Ki-67 in lung cancer. Rom J Morphol Embryol.59(1):227-233.

Das D, Sengupta M, Basu K, Tirkey M, Datta C, Chatterjee U. (2018) Role of p16/Ki-67 Dual Immunostaining in Detection of Cervical Cancer Precursors. J Cytol. Jul-Sep;35(3):153-158.

Western blot of serum starved then re-fed for 2.5 hr T47D cell lysate showing specific immunolabeling of the ~345/395 Ki-67 isoforms.
Immunostaining of HeLa cells showing specific labeling of Ki-67 in red and B-tubulin in green. DNA is stained blue.