Ki-67 Antibody

Catalog #: 1006-Ki67 Categories: , Datasheet:

$99.00$310.00

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Excellent marker for cell growth!

Formulation:
Totaly IgY fraction
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:5000IHC 1:1000-1:5000 ICC 1:1000-1:5000
Species:
Chicken Polyclonal
Gene Name:
MKI67
Molecular Weight:
~345/395
Cite This Antibody:
PhosphoSolutions Cat# 1006-Ki67: RRID:AB_2737605
Antigen/Purification: ExpandCollapse

The antigen is recombinant human Ki-67 purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Ki-67 is a nuclear protein that is  considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000).  Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).

Synonyms: ExpandCollapse

• Antigen identified by monoclonal antibody Ki 67 antibody
• Antigen identified by monoclonal antibody Ki-67 antibody
• Antigen KI-67 antibody
• Antigen KI67 antibody
• Antigen Ki67 antibody
• Ki67 antibody
• KI67_HUMAN antibody
• KIA antibody
• Marker of proliferation Ki-67 antibody
• MIB 1 antibody
• MIB antibody
• MKI67 antibody
• PPP1R105 antibody
• Proliferation marker protein Ki-67 antibody
• Proliferation related Ki 67 antigen antibody
• Protein phosphatase 1 regulatory subunit 105 antibody
• RP11-380J17.2 antibody

Storage

100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Scholzen T, Gerdes J. (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol. Mar;182(3):311-22

Yerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA (2010). Ki67 in breast cancer: prognostic and predictive potential. The Lancet. Oncology. 11 (2): 174–83.

Folescu R, Levai CM, Grigora? ML, Arghirescu TS, Talpo? IC, Gîndac CM, Zamfir CL, Poroch V, Anghel MD. (2018) Expression and significance of Ki-67 in lung cancer. Rom J Morphol Embryol.59(1):227-233.

Das D, Sengupta M, Basu K, Tirkey M, Datta C, Chatterjee U. (2018) Role of p16/Ki-67 Dual Immunostaining in Detection of Cervical Cancer Precursors. J Cytol. Jul-Sep;35(3):153-158.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of serum starved then re-fed for 2.5 hr T47D cell lysate showing specific immunolabeling of the ~345/395 Ki-67 isoforms.

Click here to view our Western blotting and lysate preparation protocols.
Immunostaining of HeLa cells showing specific labeling of Ki-67 (catalog #1007-Ki67, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.

Download the protocol used for this image:

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