Alpha Synuclein Antibody

Catalog #: 107-ASYN Categories: , , Datasheet:
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$119.00 – $380.00

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Great for ICC staining!

Formulation:
Protein G purified
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:1000ICC 1:500 Don't see your application?
Species:
Mouse
Gene Name:
SNCA
Molecular Weight:
~15 kDa
Cite This Antibody:
PhosphoSolutions Cat# 107-ASYN, RRID:AB_2492037
Antigen/Purification: ExpandCollapse

The antigen is a full length human alpha synuclein expressed in E. Coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Early onset Parkinson’s disease may be caused by a duplication or triplication of one of the alpha synuclein genes (ChartierHarlin MC et al., 2004 and Singleton, AB et al., 2005). Alpha-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy (MSA) and amyotrophic lateral sclerosis (ALS).

Synonyms: ExpandCollapse
  • Alpha synuclein antibody
  • Alpha-synuclein antibody
  • Alpha-synuclein, isoform NACP140 antibody
  • alphaSYN antibody
  • MGC105443 antibody
  • MGC110988 antibody
  • MGC127560 antibody
  • MGC64356 antibody
  • NACP antibody
  • Non A beta component of AD amyloid antibody
  • Non A4 component of amyloid antibody
  • Non A4 component of amyloid precursor antibody
  • Non-A beta component of AD amyloid antibody
  • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
  • Non-A4 component of amyloid precursor antibody
  • Non-A4 component of amyloid, precursor of antibody
  • OTTHUMP00000218549 antibody
  • OTTHUMP00000218551 antibody
  • OTTHUMP00000218552 antibody
  • OTTHUMP00000218553 antibody
  • OTTHUMP00000218554 antibody
  • PARK 1 antibody
  • PARK 4 antibody
  • PARK1 antibody
  • PARK4 antibody
  • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
  • Parkinson disease familial 1 antibody
  • SNCA antibody
  • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
  • SYN antibody
  • Synuclein alpha antibody
  • Synuclein alpha 140 antibody
  • Synuclein, alpha (non A4 component of amyloid precursor) antibody
  • SYUA_HUMAN antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat Neurons and Glia.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Okochi, M., Walter, J., Koyama, A., Nakajo, S., Baba, M., Iwatsubo, T., Meijer, L., Kahle, P.J. and Haass, C., 2000. Constitutive phosphorylation of the Parkinson’s disease associated α-synuclein. Journal of Biological Chemistry, 275(1), pp.390-397. PMID: 10617630

Chartier-Harlin, M.C., Kachergus, J., Roumier, C., Mouroux, V., Douay, X., Lincoln, S., Levecque, C., Larvor, L., Andrieux, J., Hulihan, M. and Waucquier, N., 2004. α-synuclein locus duplication as a cause of familial Parkinson’s disease. The Lancet, 364(9440), pp.1167-1169. PMID: 15451224

Singleton, A.B., Farrer, M., Johnson, J., Singleton, A., Hague, S., Kachergus, J., Hulihan, M., Peuralinna, T., Dutra, A., Nussbaum, R. and Lincoln, S., 2003. α-Synuclein locus triplication causes Parkinson’s disease. Science, 302(5646), pp.841. PMID: 14593171

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Alpha Synuclein Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~ 15 kDa alpha synuclein protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence of mixed neuron-glial cultures co-labeled with anti-alpha synuclein (cat # 107-ASYN, 1:500, red) and anti-MAP2 (cat #1100-MAP2,1:2,500, green) and nuclear staining with DAPI. The alpha-synuclein antibody labels vesicular structures, the perikarya and processes of the neurons in this image.

Download the protocol used for this image:

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