Alpha Internexin (NF66) Antibody, Chicken

Catalog #: 101-AIN Categories: , , Datasheet:

$99.00$310.00

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Great for IHC and ICC staining of neuronal processes!

Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Species Reactivity:
Most mammals
Applications:
WB 1:5000IHC 1:1000-1:5000ICC 1:500-1:1000
Species:
Chicken polyclonal
Gene Name:
INA
Molecular Reference:
~ 66 kDa
Cite This Antibody:
PhosphoSolutions Cat# 101-AIN, RRID:AB_2492033
Antigen/Purification: ExpandCollapse

The antigen is a  recombinant rat alpha-internexin expressed in and purified from E. coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alphainternexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alphainternexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).

Synonyms: ExpandCollapse
  • 66 kDa neurofilament protein antibody
  • AINX_HUMAN antibody
  • Alpha Inx antibody
  • Alpha-internexin antibody
  • Alpha-Inx antibody
  • INA antibody
  • Internexin neuronal intermediate filament protein alpha antibody
  • MGC12702 antibody
  • NEF 5 antibody
  • NEF5 antibody
  • Neurofilament 5 (66kD) antibody
  • Neurofilament 5 antibody
  • Neurofilament 66 antibody
  • Neurofilament 66 tax binding protein antibody
  • Neurofilament-66 antibody
  • NF 66 antibody
  • NF-66 antibody
  • NF66 antibody
  • TXBP 1 antibody
  • TXBP1 antibody

Storage

100 µl liquid containing 10mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat Neurons and Glia.

Immunohistochemistry

Rat Cerebellum.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).

McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002).

Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004).

Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004).

Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).

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Alpha Internexin (NF66) Antibody, Chicken
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66 kDa alpha internexin protein.

Click here to view our Western blotting and lysate preparation protocols.
Alpha Internexin (NF66) Antibody, Chicken
Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal processes with Anti-alpha-internexin (catalog # 101-AIN, 1:500, red), and specific labeling of microglia with anti-coronin 1a (green).

Download the protocol used for this image:
Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (cat #101-AIN, 1:5000, red), labeling of the myelin sheaths around the axons with MBP (cat #1120-MBP, 1:5000, green), and additional nuclear staining with DAPI (blue).

Download the protocol used for this image:

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