Alpha II Spectrin Antibody, Rabbit

Catalog #: 99-A2SR Categories: , , , Datasheet:

$99.00$310.00

  • SizePrice
Clear

Great for use in IHC and ICC staining!

Formulation:
Neat serum
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:5000IHC 1:500-1:1000ICC 1:500-1:1000
Species:
Rabbit polyclonal
Gene Name:
SPTAN1
Molecular Weight:
~240 kDa
Cite This Antibody:
PhosphoSolutions Cat# 99-A2SR, RRID:AB_2492031
Antigen/Purification: ExpandCollapse

The antigen is a recombinant construct containing the seventh, eighth and ninth repeats of human alpha II spectrin expressed in and purified from E. Coli.

The antibody is neat serum.

Biological Significance: ExpandCollapse

The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (?1 and ?2) and five beta genes (?1-?5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).

Synonyms: ExpandCollapse
  • (ALPHA)II-SPECTRIN antibody
  • Alpha-II spectrin antibody
  • brain antibody
  • EIEE5 antibody
  • FLJ17738 antibody
  • FLJ44613 antibody
  • Fodrin antibody
  • Fodrin alpha chain antibody
  • Fodrin, alpha antibody
  • NEAS antibody
  • Non erythrocytic spectrin alpha antibody
  • non-erythroid alpha chain antibody
  • SPECA antibody
  • Spectrin alpha chain antibody
  • Spectrin alpha chain brain antibody
  • Spectrin alpha non erythrocytic 1 antibody
  • Spectrin antibody
  • Spectrin non erythroid alpha chain antibody
  • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
  • Spectrin, nonerythroid, alpha subunit antibody
  • Spna2 antibody
  • SPTA 2 antibody
  • SPTA2 antibody
  • SPTA2_HUMAN antibody
  • SPTAN 1 antibody
  • SPTAN1 antibody

Storage

100 µl neat serum. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocol

Immunocytochemistry

Rat Neurons and Glia.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Bennett V & Baines AJ.(2001) Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiol Rev. 81:1353-92.

Eber S & Lux SE. (2004) Hereditary spherocytosis–defects in proteins that connect the membrane skeleton to the lipid bilayer. Semin Hematol 41:118-41.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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