Alpha II Spectrin Antibody, Mouse

Catalog #: 98-A2SM Categories: , , Datasheet:
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$109.00 – $365.00

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Excellent for use in IHC and ICC!

 

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:3000IHC 1:500-1:2000ICC 1:500-1:2000
Species:
Mouse monoclonal
Gene Name:
SPTAN1
Molecular Weight:
~240 kDa
Cite This Antibody:
PhosphoSolutions Cat# 98-A2SM, RRID:AB_2492030
Antigen/Purification: ExpandCollapse

The antigen is a recombinant construct containing the seventh, eighth and ninth repeats of human alpha II spectrin expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (?1 and ?2) and five beta genes (?1-?5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).

Synonyms: ExpandCollapse
  • (ALPHA)II-SPECTRIN antibody
  • Alpha-II spectrin antibody
  • brain antibody
  • EIEE5 antibody
  • FLJ17738 antibody
  • FLJ44613 antibody
  • Fodrin antibody
  • Fodrin alpha chain antibody
  • Fodrin, alpha antibody
  • NEAS antibody
  • Non erythrocytic spectrin alpha antibody
  • non-erythroid alpha chain antibody
  • SPECA antibody
  • Spectrin alpha chain antibody
  • Spectrin alpha chain brain antibody
  • Spectrin alpha non erythrocytic 1 antibody
  • Spectrin antibody
  • Spectrin non erythroid alpha chain antibody
  • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
  • Spectrin, nonerythroid, alpha subunit antibody
  • Spna2 antibody
  • SPTA 2 antibody
  • SPTA2 antibody
  • SPTA2_HUMAN antibody
  • SPTAN 1 antibody
  • SPTAN1 antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocol

Immunocytochemistry- Rat Neurons and Glia

Product specific protocol using rat neurons and glia.

Immunohistochemistry- Rat Cerebellum

Product specific protocol using rat cerebellum tissue.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation

General References

Bennett V & Baines AJ.(2001) Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiol Rev. 81:1353-92.

Eber S & Lux SE. (2004) Hereditary spherocytosis–defects in proteins that connect the membrane skeleton to the lipid bilayer. Semin Hematol 41:118-41.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Alpha II Spectrin Antibody, Mouse
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Click here to view our Western blotting and lysate preparation protocols.
Alpha II Spectrin Antibody, Mouse
Immunofluorescence of cultured neurons and glia cells showing specific axonal and dendritic labeling with anti-alpha-II-spectrin (catalog # 98-A2SM, 1:500, green), and nuclear staining with DAPI (blue).

Download the protocol used for this image:
Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (cat #98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (cat #621-GFAP, 1:5000, red).

Download the protocol used for this image:

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