Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- WB 1:500
- Gene Name:
- Molecular Reference:
- ~68 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p104-342, RRID:AB_2492036
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr342 of Drosophila AKT.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).