Affinity purified from pooled serum. Learn more.

AKT (Thr342) Antibody

Catalog #: p104-342 Category: Datasheet:


  • SizePrice

Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Reactivity:
WB 1:500
Gene Name:
Molecular Reference:
~68 kDa
Cite This Antibody:
PhosphoSolutions Cat# p104-342, RRID:AB_2492036
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr342 of Drosophila AKT.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (?, ?, and ?) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Akt? occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).

Synonyms: ExpandCollapse
  • AKT1 antibody
  • AKT1_HUMAN antibody
  • AKT2 antibody
  • AKT3 antibody
  • PKB alpha antibody
  • PKB antibody
  • PKB beta antibody
  • PKBalpha antibody
  • PRKBA antibody
  • PRKBB antibody
  • PRKBG antibody
  • Protein kinase B antibody
  • Protein kinase B beta antibody
  • Protein kinase B gamma antibody
  • Proto-oncogene c-Akt antibody
  • RAC alpha antibody
  • RAC antibody
  • RAC gamma antibody
  • RAC PK alpha antibody
  • RAC PK beta antibody
  • RAC PK gamma antibody
  • RAC-alpha serine/threonine-protein kinase antibody
  • RAC-PK-alpha antibody
  • STK2 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Post, S., Karashchuk, G., Wade, J.D., Sajid, W., De Meyts, P. and Tatar, M., 2018. Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28;9:245. PMID: 29892262

Lin, F., Hossain, M.A., Post, S., Karashchuk, G., Tatar, M., De Meyts, P. and Wade, J.D., 2017. Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212. PMID: 29491510

Schleich S, Strassburger K, Janiesch PC, Koledachkina T, Miller KK, Haneke K, Cheng YS, Küchler K, Stoecklin G, Duncan KE, Teleman AA (2014) DENR-MCT-1 promotes translation reinitiation downstream of uORFs to control tissue growth. Nature. Aug 14;512(7513):208-12. doi: 10.1038/nature13401.  PMID: 25043021


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AKT thr342 Antibody
Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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