Affinity purified from pooled serum. Learn more.

AKT (Thr342) Antibody

We are the original manufacturer of our AKT (Thr342) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p104-342 Categories: , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
WB 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~68 kDa
Cite This Antibody:
PhosphoSolutions Cat# p104-342, RRID:AB_2492036
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr342 of Drosophila AKT.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).

Synonyms: ExpandCollapse
  • AKT1 antibody
  • AKT1_HUMAN antibody
  • AKT2 antibody
  • AKT3 antibody
  • PKB alpha antibody
  • PKB antibody
  • PKB beta antibody
  • PKBalpha antibody
  • PRKBA antibody
  • PRKBB antibody
  • PRKBG antibody
  • Protein kinase B antibody
  • Protein kinase B beta antibody
  • Protein kinase B gamma antibody
  • Proto-oncogene c-Akt antibody
  • RAC alpha antibody
  • RAC antibody
  • RAC gamma antibody
  • RAC PK alpha antibody
  • RAC PK beta antibody
  • RAC PK gamma antibody
  • RAC-alpha serine/threonine-protein kinase antibody
  • RAC-PK-alpha antibody
  • STK2 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Drosophila

Western Blot: Drosophila
322919521:1000 Woodling, N.S., et al. 2020 The neuronal receptor tyrosine kinase Alk is a target for longevity. Aging Cell, p.e13137.
310614701:500 Xu, Y., et al. 2019 Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific Reports, 9(1), p.6989.
298922621:1000 Post, S., et al. 2018 Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28; 9:245.
29491510not listed Lin, F., et al. 2017 Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212.
250430211:200 Schleich, S., et al. 2014 DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth. Nature. Aug 14; 512(7513):208-12.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…