Affinity purified from pooled serum. Learn more.

AKT (Thr342) Antibody

Catalog #: p104-342 Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Drosophila
Applications:
WB 1:500 Don't see your application?
Species:
Rabbit
Gene Name:
AKT1
Molecular Weight:
~68 kDa
Cite This Antibody:
PhosphoSolutions Cat# p104-342, RRID:AB_2492036
Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr342 of Drosophila AKT.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).

Synonyms: ExpandCollapse
  • AKT1 antibody
  • AKT1_HUMAN antibody
  • AKT2 antibody
  • AKT3 antibody
  • PKB alpha antibody
  • PKB antibody
  • PKB beta antibody
  • PKBalpha antibody
  • PRKBA antibody
  • PRKBB antibody
  • PRKBG antibody
  • Protein kinase B antibody
  • Protein kinase B beta antibody
  • Protein kinase B gamma antibody
  • Proto-oncogene c-Akt antibody
  • RAC alpha antibody
  • RAC antibody
  • RAC gamma antibody
  • RAC PK alpha antibody
  • RAC PK beta antibody
  • RAC PK gamma antibody
  • RAC-alpha serine/threonine-protein kinase antibody
  • RAC-PK-alpha antibody
  • STK2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Drosophila


Western Blot: Drosophila
PMID Dilution Publication
31061470 1:500 Xu, Y., et al. 2019 Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific reports, 9(1), p.6989.
29892262 1:1000 Post, S., et al. 2018 Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28;9:245.
29491510 not listed Lin, F., et al. 2017 Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212.
25043021 1:200 Schleich, S., et al. 2014 DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth. Nature. Aug 14;512(7513):208-12.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

AKT thr342 Antibody
Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Click here to view our Western blotting and lysate preparation protocols.

You may also like…