Affinity purified from pooled serum. Learn more.

AIF1/Iba1 Antibody

We are the original manufacturer of our AIF1/Iba1 rabbit polyclonal antibody. It is an excellent microglia marker.

Catalog #: 91-AIF1 Categories: , Datasheet:


  • SizePrice
Pooled Serum
100 µl
Antigen affinity purified from pooled serum
Species Tested:
Expected Reactivity:
WB 1:1000ICC 1:500
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~ 17 kDa
Cite This Antibody:
PhosphoSolutions Cat# 91-AIF1, RRID:AB_2868532
Antigen/Purification: ExpandCollapse

The antigen is full-length human AIF1/Iba1 protein expressed in and purified from E. coli.

The antibody was prepared from pooled rabbit serum by affinity purification using a column to which the protein immunogen was coupled.

Biological Significance: ExpandCollapse

AIF1, allograft inflammatory factor-1,  (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).

Synonyms: ExpandCollapse



allograft inflammatory factor-1

Ionized calcium binding adaptor molecule 1


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol.

Shipped on blue ice. Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y. and Kohsaka, S., 1998. Microglia-specific localisation of a novel calcium binding protein, Iba1. Molecular Brain Research, 57(1), pp.1-9. PMID: 9630473

Watano, K., Iwabuchi, K., Fujii, S., Ishimori, N., Mitsuhashi, S., Ato, M., Kitabatake, A. and Onoé, K., 2001. Allograft inflammatory factor‐1 augments production of interleukin‐6, ‐10, and ‐12 by a mouse macrophage line. Immunology, 104(3), pp.307-316. PMID: 11722645

Romcy-Pereira, R.N., Erraji-Benchekroun, L., Smyrniotopoulos, P., Ogawa, S., Mello, C.V., Sibille, E. and Pavlides, C., 2009. Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation. Physiology & Behavior, 98(1-2), pp.44-52. PMID: 19389414

Liu, S., Tan, W.Y., Chen, Q.R., Chen, X.P., Fu, K., Zhao, Y.Y. and Chen, Z.W., 2008. Daintain/AIF‐1 promotes breast cancer proliferation via activation of the NF‐κB/cyclin D1 pathway and facilitates tumor growth. Cancer Science, 99(5), pp.952-957. PMID: 18341653

Utans, U., Arceci, R.J., Yamashita, Y. and Russell, M.E., 1995. Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection. The Journal of Clinical Investigation, 95(6), pp.2954-2962. PMID: 7769138

Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., Tokunaga, D., Hojo, T., Hamaguchi, M., Omoto, A. and Ishino, H., 2007. A critical role for allograft inflammatory factor-1 in the pathogenesis of rheumatoid arthritis. The Journal of Immunology, 178(5), pp.3316-3322. PMID: 17312183

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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