Adenylate Cyclase III Antibody

Our rabbit polyclonal Adenylate Cyclase III antibody is affinity purified, and can stain neuronal cilia. It has been optimized in WB, IHC, and ICC.

Catalog #: 85-AC3 Categories: , , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Affinity Purified
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:10,000ICC 1:500-1:10,000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
ADCY3
Molecular Weight:
~160 kDa
Cite This Antibody:
PhosphoSolutions Cat# 85-AC3, RRID:AB_2492029
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the C-terminal region of rat adenylate cyclase III conjugated to KLH.

The antibody is affinity purified rabbit serum.

Biological Significance: ExpandCollapse

Adenylate cyclase is the enzyme which produces the “second messenger” signaling molecule, cAMP from ATP. Type III adenylate cyclase is localized to the membranes surrounding neuronal cilia. Much is currently unknown about the function of primary cilia in vertebrates, however, recent work has begun to explore their role in neuronal signaling and neurogenesis (Fuchs and Schwark, 2004; Louvi and Grove 2011).

Synonyms: ExpandCollapse
  • AC-III antibody
  • AC3 antibody
  • ADCY3 antibody
  • ADCY3_HUMAN antibody
  • adenylate cyclase 3 antibody
  • Adenylate cyclase antibody
  • Adenylate cyclase type 3 antibody
  • Adenylate cyclase type III antibody
  • Adenylyl cyclase 3 antibody
  • ATP pyrophosphate lyase antibody
  • ATP pyrophosphate-lyase 3 antibody
  • olfactive type antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM NaN3.  Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Rat Neurons and Glia.

Immunohistochemistry

Rat Hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fuchs, J.L. and Schwark, H.D., 2004. Neuronal primary cilia: a review. Cell Biology International, 28(2), pp.111-118. PMID: 14984756

Louvi, A. and Grove, E.A., 2011. Cilia in the CNS: the quiet organelle claims center stage. Neuron, 69(6), pp.1046-1060. PMID: 21435552

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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