Adenylate Cyclase III Antibody

Catalog #: 85-AC3 Categories: , , Datasheet:


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Use in IHC and ICC to stain neuronal cilia!


Affinity Purified
Species Tested:
Human, Mouse, Rat
WB 1:2000IHC 1:10,000ICC 1:500-1:10,000
Rabbit polyclonal
Gene Name:
Molecular Weight:
~160 kDa
Cite This Antibody:
PhosphoSolutions Cat# 85-AC3, RRID:AB_2492029
Antigen/Purification: ExpandCollapse

The antigen is a synthetic peptide corresponding to amino acid residues from the C-terminal region of rat adenylate cyclase III conjugated to KLH.

The antibody is affinity purified rabbit serum.

Biological Significance: ExpandCollapse

Adenylate cyclase is the enzyme which produces the “second messenger” signaling molecule, cAMP from ATP. Type III adenylate cyclase is localized to the membranes surrounding neuronal cilia. Much is currently unknown about the function of primary cilia in vertebrates, however, recent work has begun to explore their role in neuronal signaling and neurogenesis (Fuchs and Schwark, 2004; Louvi and Grove 2011).

Synonyms: ExpandCollapse
  • AC-III antibody
  • AC3 antibody
  • ADCY3 antibody
  • ADCY3_HUMAN antibody
  • adenylate cyclase 3 antibody
  • Adenylate cyclase antibody
  • Adenylate cyclase type 3 antibody
  • Adenylate cyclase type III antibody
  • Adenylyl cyclase 3 antibody
  • ATP pyrophosphate lyase antibody
  • ATP pyrophosphate-lyase 3 antibody
  • olfactive type antibody


100 µl in PBS + 50% glycerol + 5 mM NaN3.  Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocol

Immunocytochemistry- Rat Neurons and Glia

Product specific protocol using rat neurons and glia.

Immunohistochemistry- Rat Hippocampus Sections

Product specific protocol using rat hippocampus.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fuchs JL, Schwark HD. (2004) Neuronal primary cilia: a review. Cell Biol Int. 28:111-8.

Louvi A and Grove EA. (2011) Cilia in the CNS: the quiet organelle claims center stage. Neuron 69:1046-1060.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Adenylate Cyclase III Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~160 kDa adenylate cyclase III protein.

Click here to view our Western blotting and lysate preparation protocols.
Adenylate Cyclase III Antibody
Immunostaining of cultured rat neurons and glia showing strong staining of neuronal cilia using our anti-adenylate cyclase III antibody (cat # 85-AC3, 1:500, red) and axonal and dendritic staining of alpha II spectrin (cat. # 98-A2SM,1:500, green) revealing the submembranous cytoskeleton and DNA (blue).

Download the protocol used for this image:
Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (cat #85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (cat #1205-MECP2 , 1:1000,green), and Hoechst staining of nuclear DNA.

Download the protocol used for this image:

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