Actin Antibody

Catalog #: 125-ACT Categories: , Datasheet:


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Mouse Monoclonal Antibody

Mouse ascites fluid
Xenopus, Drosophila, Zebra fish, Most mammals, Some invertebrates
WB 1:5,000IF 1:500IHC 1:500
Gene Name:
Molecular Reference:
~ 42 kDa
Cite This Antibody:
PhosphoSolutions Cat# 125-ACT, RRID:AB_2492035
Antigen/Purification: ExpandCollapse

The antigen is a chicken gizzard actin.

The antibody is unpurified ascites fluid.

Biological Significance: ExpandCollapse

Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.


Liquid containing 0.01% sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific References

Aten, S., Hansen, K.F., Snider, K., Wheaton, K., Kalidindi, A., Garcia, A., Alzate-Correa, D., Hoyt, K.R. and Obrietan, K., 2018. miR-132 couples the circadian clock to daily rhythms of neuronal plasticity and cognition. Learning & Memory, 25(5), pp.214-229. PMID: 29661834

Wheaton, K., Aten, S., Queiroz, L.S., Sullivan, K., Oberdick, J., Hoyt, K.R. and Obrietan, K., 2018. Circadian expression and functional characterization of PEA‐15 within the mouse suprachiasmatic nucleus. European Journal of Neuroscience. Apr;47(7):845-857. PMID: 29383758

Choi, Y.S., Horning, P., Aten, S., Karelina, K., Alzate-Correa, D., Arthur, J.S.C., Hoyt, K.R. and Obrietan, K., 2017. Mitogen-and Stress-Activated Protein Kinase 1 Regulates Status Epilepticus-Evoked Cell Death in the Hippocampus. ASN neuro, 9(5), p.1759091417726607. PMID: 28870089

Barcus, C.E., O’Leary, K.A., Brockman, J.L., Rugowski, D.E., Liu, Y., Garcia, N., Yu, M., Keely, P.J., Eliceiri, K.W. and Schuler, L.A., 2017. Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells. Breast Cancer Research, 19(1), p.9. PMID: 28103936

Ramena, G., Yin, Y., Yu, Y., Walia, V., & Elble, R. C. (2016). CLCA2 interactor EVA1 is required for mammary epithelial cell differentiation. PloS one, 11(3), e0147489. PMID: 26930581

Goodfellow, M. J., Abdulla, K. A., & Lindquist, D. H. (2016). Neonatal ethanol exposure impairs trace fear conditioning and alters NMDA receptor subunit expression in adult male and female rats. Alcoholism: Clinical and Experimental Research, 40(2), 309-318. PMID: 26842250

Barcus, C. E., Holt, E. C., Keely, P. J., Eliceiri, K. W., & Schuler, L. A. (2015). Dense Collagen-I Matrices Enhance Pro-Tumorigenic Estrogen-Prolactin Crosstalk in MCF-7 and T47D Breast Cancer Cells. PloS one, 10(1) e0116891. PMID: 25607819

Actin Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein.

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