Actin Antibody

Catalog #: 125-ACT Categories: , Datasheet:

$99.00$310.00

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Mouse Monoclonal Antibody

Formulation:
Mouse ascites fluid
Species Tested:
Human, Mouse, Rat
Species Reactivity:
Most mammals, Some invertebrates, Drosophila, Xenopus, Zebra fish
Applications:
WB 1:5000IHC 1:500ICC 1:200-1:500 Don't see your application?
Species:
Mouse
Gene Name:
ACTA1
Molecular Weight:
~ 42 kDa
Cite This Antibody:
PhosphoSolutions Cat# 125-ACT, RRID:AB_2492035
Antigen/Purification: ExpandCollapse

The antigen is a chicken gizzard actin.

The antibody is unpurified ascites fluid.

Biological Significance: ExpandCollapse

Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Synonyms: ExpandCollapse
  • a actin antibody
  • ACTA antibody
  • ACTA1 antibody
  • Actin alpha skeletal muscle antibody
  • Actin antibody
  • actin, alpha 1, skeletal muscle 1 antibody
  • actin, alpha 1, skeletal muscle antibody
  • Actin, alpha skeletal muscle antibody
  • actina antibody
  • actine antibody
  • ACTS_HUMAN antibody
  • aktin antibody
  • Alpha Actin 1 antibody
  • Alpha skeletal muscle Actin antibody
  • alpha skeletal muscle antibody
  • alpha-actin antibody
  • Alpha-actin-1 antibody
  • ASMA antibody
  • CFTD antibody
  • CFTD1 antibody
  • CFTDM antibody
  • MPFD antibody
  • NEM1 antibody
  • NEM2 antibody
  • NEM3 antibody
  • nemaline myopathy type 3 antibody

Storage

100 µl liquid containing 0.01% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western Blot: Human
PMID Dilution Publication
28103936 not listed Barcus, C.E., et al. 2017 Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells. Breast Cancer Research, 19(1), p.9.
26930581 not listed Ramena, G., et al. 2016 CLCA2 interactor EVA1 is required for mammary epithelial cell differentiation. PloS one, 11(3), e0147489.
25607819 not listed Barcus, C.E., et al. 2015 Dense Collagen-I Matrices Enhance Pro-Tumorigenic Estrogen-Prolactin Crosstalk in MCF-7 and T47D Breast Cancer Cells. PloS one, 10(1) e0116891.


Western Blot: Mouse
PMID Dilution Publication
29661834 1:100,000 Aten, S., et al. 2018 miR-132 couples the circadian clock to daily rhythms of neuronal plasticity and cognition. Learning & Memory, 25(5), pp.214-229.
29383758 1:100,000 Wheaton, K., et al. 2018 Circadian expression and functional characterization of PEA‐15 within the mouse suprachiasmatic nucleus. European Journal of Neuroscience, Apr;47(7):845-857.
28870089 1:1000 Choi, Y.S., et al. 2016 Mitogen-and Stress-Activated Protein Kinase 1 Regulates Status Epilepticus-Evoked Cell Death in the Hippocampus. ASN neuro, 9(5), p.1759091417726607.


Western Blot: Rat
PMID Dilution Publication
26842250 1:1500 Goodfellow, M.J., et al. 2016 Neonatal ethanol exposure impairs trace fear conditioning and alters NMDA receptor subunit expression in adult male and female rats. Alcoholism: Clinical and Experimental Research, 40(2), 309-318.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Actin Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence staining of human blood-brain barrier endothelial cells, HMEC 3, showing specific labeling of actin (cat# 125-ACT, red, 1:200) The cell nuclei are stained in blue with DAPI. Photo credit of Yancy Ferrer-Acosta/Henrique Martins, Universidad Central Del Caribe, PR.

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