Affinity purified from pooled serum. Learn more.

5-Lipoxygenase (Ser523) Antibody

Catalog #: p175-523 Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Species Reactivity:
Non-human Primate
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
ALOX5
Molecular Weight:
~80 kDa
Cite This Antibody:
PhosphoSolutions Cat# p175-523, RRID:AB_2492028
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-lipoxygenase (5-LO).

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Synonyms: ExpandCollapse
  • 5 Lipoxygenase antibody
  • 5 LO antibody
  • 5 LOX antibody
  • 5-lipoxygenase antibody
  • 5-LO antibody
  • 5-LOX antibody
  • 5LOX antibody
  • 5LPG antibody
  • ALOX 5 antibody
  • Alox5 antibody
  • Arachidonate 5 lipoxygenase antibody
  • Arachidonate 5-lipoxygenase antibody
  • arachidonic 5-lipoxygenase alpha-10 isoform antibody
  • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
  • arachidonic 5-lipoxygenase delta-13 isoform antibody
  • arachidonic 5-lipoxygenase delta-p10 isoform antibody
  • Arachidonic acid 5 lipoxygenase antibody
  • Leukotriene A4 synthase antibody
  • LOG 5 antibody
  • LOG5 antibody
  • LOX5_HUMAN antibody
  • MGC163204 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
15280375 not listed Luo, M., et al. 2004 Luo, M., Jones, S.M., Phare, S.M., Coffey, M.J., Peters-Golden, M. and Brock, T.G., 2004. Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523. Journal of Biological Chemistry, 279(40), pp.41512-41520.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

5-Lipoxygenase ser523 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

You may also like…