Anti-14-3-3 (Ser58) Antibody

Name: Anti-14-3-3 (Ser58) Antibody
Brand: PhosphoSolutions
SKU: p1433-58
Price: 430.0 USD
Availability: InStock

2 Citations

Our Anti-14-3-3 (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 14-3-3 (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).

Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58(cat. p1433-58, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

Species Reactivity

Human, Mouse, Rat, Salamander

Applications

IHC, WB

Host Species

Rabbit

Go to Full Product Details

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

SKU: p1433-58

Volume: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

14-3-3 (Ser58)

Target Description

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

YWHAB

Molecular Weight

29 kDa

Antigen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat, Salamander

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate, Sheep, Xenopus, Zebrafish

Antibody Registry ID

AB_2492027

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~29 kDa 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser58

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

14 3 3 antibody, 14 3 3 protein beta antibody, 14 3 3 protein beta/alpha antibody, 14 3 3 protein zeta antibody ,14 3 3 zeta antibody,14-3-3 protein beta/alpha antibody, 14-3-3 protein/cytosolic phospholipase A2 antibody, 1433B_HUMAN antibody, GW128 antibody, HS1 antibody, KCIP 1 antibody, KCIP-1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein delta polypeptide antibody, Tyrosine 3/tryptophan 5 -monooxygenase activation protein zeta polypeptide antibody, YWHAB antibody, YWHAD antibody, YWHAZ antibody

UniProt Details

UniProt (Immunogen Species): P35213

NCBI Gene ID

56011

Product Specific References for Applications and Species

Immunohistochemistry: rat
Western Blot: rat

Immunohistochemistry: Rat
PMID	Dilution	Publication
18296656	1:100	Yang, W., et al. 2008. Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 - 2494.

Western Blot: Rat
PMID	Dilution	Publication
18296656	1:1000	Yang, W., et al. 2008. Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 - 2494.

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-14-3-3 (Ser58) Antibody

Our Anti-14-3-3 (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 14-3-3 (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

SKU: p1433-58

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-14-3-3 (Ser58) Antibody

Product Specific Protocols

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