Affinity purified from pooled serum. Learn more.

14-3-3 Protein (Ser58) Antibody

Catalog #: p1433-58 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat, Salamander
Species Reactivity:
Bovine, Canine, Chicken, Non-human primate, Sheep, Xenopus, Zebra fish
Applications:
WB 1:1000IHC 1:100-1:500 Don't see your application?
Species:
Rabbit
Gene Name:
YWHAB
Molecular Weight:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1433-58, RRID:AB_2492027
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser58 of rat 14-3-3 protein.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

 

Biological Significance: ExpandCollapse

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)

Synonyms: ExpandCollapse
  • 14 3 3 antibody
  • 14 3 3 protein beta antibody
  • 14 3 3 protein beta/alpha antibody
  • 14 3 3 protein zeta antibody
  • 14 3 3 zeta antibody
  • 14-3-3 protein beta/alpha antibody
  • 14-3-3 protein/cytosolic phospholipase A2 antibody
  • 1433B_HUMAN antibody
  • GW128 antibody
  • HS1 antibody
  • KCIP 1 antibody
  • KCIP-1 antibody
  • MGC111427 antibody
  • MGC126532 antibody
  • MGC138156 antibody
  • N-terminally processed antibody
  • Protein 1054 antibody
  • Protein kinase C inhibitor protein 1 antibody
  • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide antibody
  • Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide antibody
  • YWHAB antibody
  • YWHAD antibody
  • YWHAZ antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: rat
Western Blot: rat


Immunohistochemistry: Rat
PMID Dilution Publication
18296656 1:100 Yang, W., et al. 2008 Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 – 2494.


Western Blot: Rat
PMID Dilution Publication
18296656 1:1000 Yang, W., et al. 2008 Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 – 2494.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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14-3-3 Protein ser58 Antibody
Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).

Click here to view our Western blotting and lysate preparation protocols.
14-3-3 Protein ser58 Antibody
Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58(cat# p1433-58, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

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