Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- Bovine, Canine, Chicken, Human, Mouse, Non-human primate, Rat, Sheep, Xenopus, Zebra fish
- WB 1:1000IHC 1:500
- Gene Name:
- Molecular Reference:
- ~29 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1433-58, RRID:AB_2492027
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser58 of rat 14-3-3 protein.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: ExpandCollapse
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)