Protein ubiquitination has been implicated recently in neural development, plasticity, and degeneration. ZNRF1/nin283, is a protein with a unique, evolutionarily conserved C-terminal domain containing a juxtaposed zinc finger/RING finger combination. ZNRF2, is another novel member of the family of ZNRF E3 ubiquitin ligases. Both ZNRF1 and ZNRF2 have E3 ubiquitin ligase activity and are highly expressed in the nervous system, particularly during development. In neurons, ZNRF proteins are located in different compartments within the presynaptic terminal: ZNRF1 is associated with synaptic vesicle membranes, whereas ZNRF2 is present in presynaptic plasma membranes. Data suggest that ZNRF proteins play a role in the establishment and maintenance of neuronal transmission and plasticity via their ubiquitin ligase activity.
Antigen Affinity Purified
Synthetic peptide corresponding to amino acids from an internal region of human DPP10 protein
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
PBS + 0.02% NaN3
WB Brain: 1:500-1:1000
Reacts with an internal region of the 24 kDa human ZNRF2 protein. 87% sequence identity to rat and 81% sequence identity to mouse.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
E3 ubiquitin-protein ligase ZNRF2, Protein Ells2, RING finger protein 202, Zinc/RING finger protein 2, RING-type E3 ubiquitin transferase