Name: Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Antibody
Brand: PhosphoSolutions
SKU: p2400-295
Price: 415.0 USD
Availability: InStock

Our Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse

Expected Reactivity

Canine, Human, Non-Human Primate, Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

XIRP1

Molecular Weight

150, 250 kDa

Go to Full Product Details

Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser295 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Antibody

Product Details

Target

Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295)

Target Description

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

XIRP1

Molecular Weight

150, 250 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse

Expected Reactivity

Canine, Human, Non-Human Primate, Rat

Antibody Registry ID

AB_2492287

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~250 kDa and ~150 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser295

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AI415219 antibody, Cardiomyopathy-associated protein 1 antibody, CMYA1 antibody, DKFZp451D042 antibody, DKFZp779C1255 antibody, DKFZp779C1947 antibody, MGC144145 antibody, MGC144146 antibody, Xin actin-binding repeat-containing protein 1 antibody, XIN antibody, Xirp1 antibody, XIRP1_HUMAN antibody

UniProt Details

UniProt (Human): Q702N8
UniProt (Immunogen Species): O70373

NCBI Gene ID

165904

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
23882026	not listed	Chang, Y.W., et al. 2013. Quantitative phosphoproteomic study of pressure-overloaded mouse heart reveals dynamin-related protein 1 as a modulator of cardiac hypertrophy. Molecular & Cellular Proteomics, 12(11), 3094-3107.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Antibody