Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WAVE2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).
Bulk Order Anti-WAVE2 (Tyr-150) [conserved site], Phosphospecific Antibody
Product Details
WAVE2 (Tyr-150)
The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
WASF2
78
Phospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:200
Unconjugated
This antibody was cross-adsorbed to phospho-WAVE (Tyr-125) and unphosphorylated WAVE (Tyr-150) peptides before affinity purification using phospho-WAVE (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa* corresponding to phosphorylated WAVE2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.
Phosphorylated
Tyr-150
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
