Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).
Anti-WAVE1 (Tyr-125), Phosphospecific Antibody
Bulk Order Anti-WAVE1 (Tyr-125), Phosphospecific Antibody
The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
Antigen Affinity Purified
Phospho-WAVE (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
This antibody was cross-adsorbed to phospho-WAVE (Tyr-150) and unphosphorylated WAVE (Tyr-125) peptides before affinity purification using phospho-WAVE (Tyr-125) peptide. In western blots, the antibody detects an 80 kDa* band corresponding to phosphorylated WAVE in human SYF cSrc transformed cells and mouse macrophages treated with pervanadate, but is not observed in control cells. These bands can be specifically blocked with phospho-WAVE (Tyr-125) peptide (WX1775), but not phospho-WAVE (Tyr-150) peptide (WX1825).
Western blots performed on each lot.
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After date of receipt, stable for at least 1 year at -20°C.