Anti-WASP / N-WASP Antibody

Name: Anti-WASP / N-WASP Antibody
Brand: PhosphoSolutions
SKU: WP2101
Price: 329.0 USD
Availability: InStock

1 Citation

Our WASP / N-WASP rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat WASP / N-WASP and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (WP2101) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis of Jurkat (lane 1), A431 (lane 2), and HeLa (lane 3) cell lysates (20 µg/lane). Blots were probed with rabbit polyclonal anti-WASP/N-WASP (WP2101).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: WP2101

Product Details

Target

WASP / N-WASP

Target Description

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

WASL

Molecular Weight

Antigen

A synthetic peptide (coupled to KLH) corresponding to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 63 kDa* protein corresponding to the molecular mass of WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Neural Wiskott-Aldrich syndrome protein, WASL, WASP

UniProt Details

UniProt (Human): O00401
UniProt (Immunogen Species): O00401

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28231468	not listed	Cao, M, et al. 2017. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron, 882-896.

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Anti-WASP / N-WASP Antibody

SKU: WP2101

Product Details

Product Specific References for Applications and Species

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