Anti-Vimentin Antibody (2D1)

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Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Vimentin and is Protein G purified. It is great for use in WB, IHC, ICC.

Primary Antibody
Bovine, Human, Mouse, Rat
Bovine, Canine, Chicken, Drosophila, Feline, Finch, Fish, Goat, Guinea Pig, Hamster, Horse, Lizard, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus, Zebrafish
2D1
ICC, IHC, WB
Mouse
Protein G Purified
VIM
50 kDa

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).

Anti-Vimentin Antibody
SKU: 2107-VIM

Volume: 100 µL
Price:
$415

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