Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Vimentin and is Protein G purified. It is great for use in WB, IHC, ICC.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Protein G purified culture supernatant.
PBS + 10 mM NaN3.
WB: 1:1000
IHC: 1:500-1:1000
ICC: 1:500-1:2000
Unconjugated
Specific for endogenous levels of the ~50 kDa vimentin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody
Nieminen, M., Henttinen, T., Merinen, M., Marttila–Ichihara, F., Eriksson, J.E. and Jalkanen, S., 2006. Vimentin function in lymphocyte adhesion and transcellular migration. Nature Cell Biology, 8(2), pp.156-162. PMID: 16429129
Zhao, Y., Yan, Q., Long, X., Chen, X. and Wang, Y., 2008. Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochemistry and Function: Cellular Biochemistry and Its Modulation by Active Agents or Disease, 26(5), pp.571-577. PMID: 18464297
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