Name: Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody
Brand: PhosphoSolutions
SKU: 2250-VMAT2C
Price: 415.0 USD
Availability: InStock

Our Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Vesicular Monoamine Transporter 2, C-terminus (VMAT2) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Applications

IHC, WB

Host Species

Sheep

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

VMAT2

Molecular Weight

57 kDa

Go to Full Product Details

Immunostaining against ChAT (red) and VMAT2 (Cat no. 2250-VMAT2C, 1:200, green) in retinal sections of control (e) and Parkinson's Desease (f) patients. Image from publication CC-BY-4.0. PMID: 37013599

Retinal sections immunostained against TH (red), ChAT (green) and vesicular monoamine transporter 2 (VMAT2, blue). S1/S2: stratum 1 and 2 in the IPL. S3/S4: stratum 3 and 4 in the IPL. Scale bar: 20 μm. Image from publication CC-BY-4.0. PMID: 37013599

Western blot of rat striatal lysate showing specific immunolabeling of the ~57 kDa VMAT2 protein.

Volume: 100 µL

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Price:

$415

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Bulk Order Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody

Product Details

Target

Vesicular Monoamine Transporter 2, C-terminus (VMAT2)

Target Description

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Sheep

Gene Name

VMAT2

Molecular Weight

57 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region of human VMAT2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Rat

Antibody Registry ID

AB_2492286

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~57 kDa VMAT2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

1110037L13Rik antibody, 9330105E13 antibody, MGC120477 antibody, MGC120478 antibody, MGC26538 antibody, MGC90556 antibody, MNAT antibody, Monoamine neurotransmitter transporter antibody, Monoamine transporter antibody, OTTHUMP00000020576 antibody, SLC18A2 antibody, Solute carrier family 18 (vesicular monoamine) member 2 antibody, Solute carrier family 18 member 2 antibody, SVAT antibody, SVMT antibody, Synaptic vesicle amine transporter brain antibody, Synaptic vesicle monoamine transporter brain antibody, Synaptic vesicular amine transporter antibody, VAT 2 antibody, VAT2 antibody, Vesicle monoamine transporter type 2 antibody, Vesicle monoamine/H+ antiporter antibody, Vesicular amine transporter 2 antibody, Vesicular monoamine transporter 2 antibody, VMAT 2 antibody, VMAT2 antibody, VMAT2_HUMAN antibody

UniProt Details

UniProt (Human): Q05940
UniProt (Immunogen Species): Q05940

NCBI Gene ID

6571

Product Specific References for Applications and Species

Immunohistochemistry: Human | Mouse
Western Blot: Rat

Immunohistochemistry: Human
PMID	Dilution	Publication
37013599	1:200	Sánchez-Sáez, X., et al. 2023. Starburst amacrine cells, involved in visual motion perception, loose their synaptic input from dopaminergic amacrine cells and degenerate in Parkinson's disease patients. Translational Neurodegeneration, 17.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26356010	1:10,000	Ivanova, E., et al. 2016. Disruption in Dopaminergic Innervation During Photoreceptor Degeneration. Journal of Comparative Neurology, .

Western Blot: Rat
PMID	Dilution	Publication
29729891	1:1000	Colon-Perez, L.M., et al. 2018. Functional Connectivity, Behavioral and Dopaminergic Alterations 24 Hours Following Acute Exposure to Synthetic Bath Salt Drug Methylenedioxypyrovalerone. Neuropharacology, .

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody

Our Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Vesicular Monoamine Transporter 2, C-terminus (VMAT2) and is antigen affinity purified from pooled serum. It is great for use in WB.