Anti-VE-Cadherin (Tyr-685), Phosphospecific Antibody
Our VE-Cadherin (Tyr-685) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VE-Cadherin (Tyr-685) and is antigen affinity purified. It is great for use in WB.
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).
Product Specific References for Applications and Species
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 21858121 | 1:200 | van der Heijden, M, et al. 2011. Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells. PLoS One, e23448. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 26272756 | not listed | Sivaraj, KK, et al. 2015. Endothelial Gαq/11 is required for VEGF-induced vascular permeability and angiogenesis. Cardiovascular Research, 171-180. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 31826007 | 1:1000 | Hiepen, C, et al. 2019. BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics. PLoS Biology, e3000557. |
| 27871478 | 2 ug/ml | Ebrahim, NA, et al. 2016. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms. Placenta, 87-98. |
| 27601468 | not listed | Wylezinski, LS, et al. 2016. Interleukin 2 Activates Brain Microvascular Endothelial Cells Resulting in Destabilization of Adherens Junctions. The Journal of Biological Chemistry, 22913-22923. |
| 26598555 | 1:1000 | Benn, A, et al. 2016. VE-cadherin facilitates BMP-induced endothelial cell permeability and signaling. Journal of Cell Science, 206-218. |
| 21123455 | not listed | Lo, CW, et al. 2011. IL-6 trans-signaling in formation and progression of malignant ascites in ovarian cancer. Cancer Research, 424-434. |
| 20048167 | not listed | Adam, AP, et al. 2010. Src-induced tyrosine phosphorylation of VE-cadherin is not sufficient to decrease barrier function of endothelial monolayers. The Journal of Biological Chemistry, 7045-7055. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37936984 | 1:1000 | Jin, Y, et al. 2022. Tyrosine-protein kinase Yes controls endothelial junctional plasticity and barrier integrity by regulating VE-cadherin phosphorylation and endocytosis. Nature Cardiovascular Research, 1156-1173. |
| 34376570 | 1:100 | Kataru, RP, et al. 2021. Lymphatic-specific intracellular modulation of receptor tyrosine kinase signaling improves lymphatic growth and function. Science signaling, eabc0836. |
| 33566443 | not listed | Ziogas, A, et al. 2021. Glycolysis is integral to histamine–induced endothelial hyperpermeability. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, e21425. |
| 26272756 | not listed | Sivaraj, KK, et al. 2015. Endothelial Gαq/11 is required for VEGF-induced vascular permeability and angiogenesis. Cardiovascular Research, 171-180. |
| 24487320 | not listed | Wessel, F, et al. 2014. Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nature Immunology, 223-230. |
| 18606869 | not listed | Fainaru, O, et al. 2008. Doxycycline induces membrane expression of VE-cadherin on endothelial cells and prevents vascular hyperpermeability. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 3728-3735. |