Our Vav2 (Tyr-142) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Vav2 (Tyr-142) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6).
The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
VAV2
100
Phospho-Vav2 (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-142 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-142) peptide before affinity purification using phospho-Vav2 (Tyr-142) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
Phosphorylated
Tyr-142
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
